Product Name :
MERTK/TYRO3  polyclonal antibody Background :
Receptor tyrosine kinases (RTKs) represent an important class of transmembrane signaling molecules. Binding of the extracellular domain of an RTK to its cognate ligand leads to receptor dimerization and the activation of the intrinsic tyrosine kinase activity of its intracellular kinase domain. The Axl/ UFO subfamily of receptor tyrosine kinases is comprised of members Tyro3 (also referred to as BYK, Brt, Dtk, RSE, Tif or Sky), Axl (also called Tyro7 or UFO) and Mer (also called Nyk, c-Eyk and Tyro12). Members of this family have a common molecular structure which contains an N-terminal extracellular domain comprised of two Ig domains, two FNIII domains and a membrane spanning single helix followed by the cytoplasmic tyrosine kinase domain. These RTKs are functionally significant in spermatogenesis, immunoregulation and phagocytosis. Tyro3, Axl and Mer are widely expressed in adult tissues with their expression most abundant in brain, testis, lymphatic and vascular tissue. Tyro3 has been shown to undergo posttranslational modifications including both tyrosine phosphorylation as well as glycosylation. Two proteins, protein S and Gas6, have been proposed as ligands for the Axl/UFO family of receptors. Both function as anti-coagulants through an unknown mechanism. Gas6 was cloned as a growth arrest-specific gene, while protein S is an abundant serum protein which is thought to act by indirectly inhibiting proteases involved in the coagulation response. Product :
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.3. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
MERTK/TYRO3 polyclonal antibody detects endogenous levels of MERTK/TYRO3 protein. Immunogen :
Synthetic peptide, corresponding Human MERTK/TYRO3. Conjugate :
Unconjugated Modification :
Unmodification

• 
  Western blot (WB) analysis of MERTK/TYRO3 polyclonal antibody at 1:500 dilution Lane1:HepG2 whole cell lysate(40ug) Lane2:A375 whole cell lysate(40ug) Lane3:Jurkat whole cell lysate(40ug)

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

---

Price/Size :

USD 368/1mg/vial

---

---

Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

---

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

---

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

---

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

---

Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

---