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Cyclin D1 polyclonal antibody BS6532
  • Western blot analysis of extracts of various cell lines, using CCND1 antibody at 1:1000 dilution.
Product NameCyclin D1 polyclonal antibody
Catalog No.BS6532
Swiss-ProtP24385
Host Rabbit
ReactivityHuman
ApplicationsWB
Application_allWB 1:500 - 1:2000
BiowMW~ 36 kDa
Alternative NameG1/S-specific cyclin-D1; B-cell lymphoma 1 protein; BCL-1; BCL-1 oncogene; PRAD1 oncogene; CCND1; BCL1; PRAD1
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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50ul $208
100ul $358
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Product Name :
Cyclin D1 polyclonal antibody
Background :
Considerable effort directed towards the identification of G1 cyclins has led to the isolation of cyclin D, cyclin C and cyclin E. Of these, cyclin D corresponds to a putative human oncogene, designated PRAD1, which maps at the site of the Bcl1 rearrangement in certain lymphomas and leukemias. Two additional human type D cyclins, as well as their mouse homologs, have been identified. Evidence has established that members of the cyclin D family function to regulate phosphorylation of the retinoblastoma gene product, thereby activating E2F transcription factors.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
Cyclin D1 polyclonal antibody detects endogenous levels of Cyclin D1 protein.
Immunogen :
Recombinant full length Human Cyclin D1.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot analysis of extracts of various cell lines, using CCND1 antibody at 1:1000 dilution.
The tumor-suppressor gene LZTS1 suppresses colorectal cancer proliferation through inhibition of the AKT–mTOR signaling pathway PMCID:    Pubmed No.:25667121 Long non-coding RNA LINC00968 acts as oncogene in NSCLC by activating the Wnt signaling pathway PMCID:    Pubmed No.:28926089 Hepatitis B x-interacting protein induces HepG2 cell proliferation through activation of the phosphatidylinositol 3-kinase/Akt pathway PMCID:    Pubmed No.:21239735 Lewis y Regulate Cell Cycle Related Factors in Ovarian Carcinoma Cell RMG-I in Vitro via ERK and Akt Signaling Pathways PMCID:    Pubmed No.:22312289 Methylation of TFPI-2 is an early event of esophageal carcinogenesis PMCID:    Pubmed No.:22449186 A novel role of ribonuclease inhibitor in regulation of epithelial-to-mesenchymal transition and ILK signaling pathway in bladder cancer cells PMCID:    Pubmed No.:23703635 MicroRNA‑214 suppresses the proliferation of human hepatocellular carcinoma cells by targeting E2F3 PMCID:    Pubmed No.:26535064 Inhibition of mTOR signaling protects photoreceptor cells against serum deprivation by reducing oxidative stress and inducing G2/M cell cycle arrest PMCID:    Pubmed No.:27035647 MicroRNA‑214 suppresses the proliferation of human hepatocellular carcinoma cells by targeting E2F3 PMCID:    Pubmed No.:26535064 Inhibition of mTOR signaling protects photoreceptor cells against serum deprivation by reducing oxidative stress and inducing G2/M cell cycle arrest PMCID:    Pubmed No.:27035647 Wogonoside induces growth inhibition and cell cycle arrest via promoting the expression and binding activity of GATA-1 in chronic myelogenous leukemia cells PMCID:    Pubmed No.:26104856 Aquaporin‐1 retards renal cyst development in polycystic kidney disease by inhibition of Wnt signaling PMCID:    Pubmed No.:25573755 Tumorigenic hybrids between mesenchymal stem cells and gastric cancer cells enhanced cancer proliferation, migration and stemness PMCID:    Pubmed No.:26498753 Targeted deletion of miR‐139‐5p activates MAPK, NF‐κB and STAT3 signaling and promotes intestinal inflammation and colorectal cancer PMCID:    Pubmed No.:26859226 Let‐7f Inhibits Glioma Cell Proliferation, Migration, and Invasion by Targeting Periostin PMCID:    Pubmed No.:25735962 Suppression of RAD21 Induces Senescence of MDA‐MB‐231 Human Breast Cancer Cells Through RB1 Pathway Activation Via c‐Myc Downregulation PMCID:    Pubmed No.:26529363 Water Stress Proteins from Nostoc commune Vauch. Exhibit Anti-Colon Cancer Activities in Vitro and in Vivo PMCID:    Pubmed No.:25524246 miR-422a inhibits cell proliferation in colorectal cancer by targeting AKT1 and MAPK1 PMCID:    Pubmed No.:29118671 MCM2 is a therapeutic target of lovastatin in human non-small cell lung carcinomas PMCID:    Pubmed No.:25738322 Fangchinoline as a kinase inhibitor targets FAK and suppresses FAK-mediated signaling pathway in A549 PMCID:    Pubmed No.:25539072 Psoralen promotes the expression of cyclin D1 in chondrocytes via the Wnt/β-catenin signaling pathway PMCID:    Pubmed No.:28949389 Oxymatrine targets EGFRp-Tyr845 and inhibits EGFR-related signaling pathways to suppress the proliferation and invasion of gastric cancer cells PMCID:    Pubmed No.:25527205 TPX2 Level Correlates with Hepatocellular Carcinoma Cell Proliferation, Apoptosis, and EMT PMCID:    Pubmed No.:26025609 Methylation of DACT2 Promotes Papillary Thyroid Cancer Metastasis by Activating Wnt Signaling PMCID:    Pubmed No.:25375359 Inhibition of mTOR signaling protects photoreceptor cells against serum deprivation by reducing oxidative stress and inducing G2/M cell cycle arrest PMCID:    Pubmed No.:27035647 Glycolysis inhibitor 2-deoxy-D-glucose suppresses carcinogen-induced rat hepatocarcinogenesis by restricting cancer cell metabolism PMCID:    Pubmed No.:25394852 MicroRNA‑214 suppresses the proliferation of human hepatocellular carcinoma cells by targeting E2F3 PMCID:    Pubmed No.:26788207 MiR-202 promotes endometriosis by regulating SOX6 expression PMCID:    Pubmed No.:26770366 Silencing NKD2 by promoter region hypermethylation promotes gastric cancer invasion and metastasis by up-regulating SOX18 in human gastric cancer PMCID:    Pubmed No.:26396173 Repression of PES1 expression inhibits growth of gastric cancer PMCID:    Pubmed No.:26423399 Inhibition of IL-8-mediated endothelial adhesion, VSMCs proliferation and migration by siRNA-TMEM98 suggests TMEM98's emerging role in atherosclerosis PMCID:    Pubmed No.:29152140 Pathway-focused PCR array profiling of CAL-27 cell with over-expressed ZNF750 PMCID:    Pubmed No.:29416636 Long noncoding RNA NEAT1 promotes cell proliferation, migration, and invasion in hepatocellular carcinoma through interacting with miR‐384 PMCID:    Pubmed No.:30346062 Combination of gemcitabine and erlotinib inhibits recurrent pancreatic cancer growth in mice via the JAK-STAT pathway PMCID:    Pubmed No.:29328487 miR-34a-5p inhibits the malignant progression of KSHV-infected SH-SY5Y cells by targeting c-fos PMCID:    Pubmed No.:35444864 UVB-induced SFRP1 methylation potentiates skin damage by promoting cell apoptosis and DNA damage PMCID:    Pubmed No.:35657114 Parthenolide inhibits proliferation of cells infected with Kaposi’s sarcoma-associated herpesvirus by suppression of the NF-κB signaling pathway PMCID:    Pubmed No.:36609933
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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