Contact us : info@bioworlde.com
Home > Product > Primary Antibodies
NFkB-p65 (phospho-S536) polyclonal antibody BS4138
  • Western blot (WB) analysis of p-NFKB-p65(S536) pAb at 1:500 dilution Lane1:HCT116 whole cell lysate(40ug) Lane2:A2780 whole cell lysate(40ug) Lane3:A549 whole cell lysate(40ug)
Product NameNFkB-p65 (phospho-S536) polyclonal antibody
Catalog No.BS4138
Swiss-ProtQ04206
Host Rabbit
ReactivityHuman
ApplicationsWB
Application_allWB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:50~1:200
BiowMW~ 65 kDa
Alternative Namep65; p65 NF kappaB; p65 NFkB; RELA; NFKB P65; Transcription Factor p65; Nuclear factor NF-kappa-B p65 subunit; NFkB-p65; NFKB3
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationPhosphorylation
Browse similar products>>
Size Price
50ul $168
100ul $298
Add to cart My orders
Product Name :
NFkB-p65 (phospho-S536) polyclonal antibody
Background :
p65 is a subunit of the nuclear factor kappa B. The transcription factor NFkB is widely recognized as a critical mediator of immune and inflammatory responses. In most cell types, NFkB is found in the cytoplasm where it is associated with an inhibitory prd in many tissues. A high proportion of spontaneous NIH/3T3 transformants overexpress c-Met and by transfection analysis the c-Met proto-oncogene has been shown to exhibittransforming activity.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
p-NFκB-p65 (S536) polyclonal antibody detects endogenous levels of NFκB-p65 p53 protein when phosphorylated at Ser536.
Immunogen :
Synthetic phosphopeptide derived from human NFkB-p65 around the phosphorylation site of Serine 536.
Conjugate :
Unconjugated
Modification :
Phosphorylation
  • Western blot (WB) analysis of p-NFKB-p65(S536) pAb at 1:500 dilution Lane1:HCT116 whole cell lysate(40ug) Lane2:A2780 whole cell lysate(40ug) Lane3:A549 whole cell lysate(40ug)
Ginsenoside Rg1 protects against hydrogen peroxide-induced cell death in PC12 cells via inhibiting NF-kB activation PMCID:    Pubmed No.:21078355 Maltose-binding protein isolated from Escherichia coli induces Toll-like receptor 2-mediated viability in U937 cells PMCID:    Pubmed No.:21775279 Curcumin inhibits tumor proliferation induced by neutrophil elastase through the upregulation of a1-antitrypsin in lung cancer PMCID:    Pubmed No.:22507634 Tumor Necrosis Factor Receptor Associated Factor 5 is an Essential Mediator of Ischemic Brain Infarction PMCID:    Pubmed No.:23413803 Melatonin improves short and long-term neurobehavioral deficits and attenuates hippocampal impairments after hypoxia in neonatal mice PMCID:    Pubmed No.:23917218 Lipoxin A4 attenuation of endothelial inflammation response mimicking pancreatitis-induced lung injury. PMCID:    Pubmed No.:24000382 Interferon Regulatory Factor 8 Protects Against Cerebral Ischaemic‐Reperfusion Injury PMCID:    Pubmed No.:24528256 Activation of Mesenchymal Stem Cells by Macrophages Prompts Human Gastric Cancer Growth through NF-κB Pathway PMCID:    Pubmed No.:24824968 Mitofusin 2 deficiency leads to oxidative stress that contributes to insulin resistance in rat skeletal muscle cells PMCID:    Pubmed No.:25034891 Arctigenin but not arctiin acts as the major effective constituent of Arctium lappa L. fruit for attenuating colonic inflammatory response induced by dextran sulfate sodium in mice. (Application: Mouse WB) PMCID:    Pubmed No.:25284342 Interferon Regulatory Factor 7 Protects Against Vascular Smooth Muscle Cell Proliferation and Neointima Formation. PMCID:    Pubmed No.:25304854 Rhizoma Dioscoreae Nipponicae polysaccharides protect HUVECs from H2O2-induced injury by regulating PPARγ factor and the NADPH oxidase/ROS-NF-κB signal pathway. PMCID:    Pubmed No.:25305479 Fucoidan Suppresses Hypoxia-Induced Lymphangiogenesis and Lymphatic Metastasis in Mouse Hepatocarcinoma PMCID:    Pubmed No.:26047481 Norisoboldine ameliorates DSS-induced ulcerative colitis in mice through induction of regulatory T cells in colons. PMCID:    Pubmed No.:26363976 DGAEE, a newly synthesized derivative of glycyrrhetinic acid, potently attenuates mouse septic shock via its main metabolite DGA in an IL-10-dependent manner PMCID:    Pubmed No.:26456500 Apigenin and naringenin regulate glucose and lipid metabolism, and ameliorate vascular dysfunction in type 2 diabetic rats PMCID:    Pubmed No.:26801071 The Upregulation of TRAF1 Induced by Helicobacter pylori Plays an Antiapoptotic Effect on the Infected Cells PMCID:    Pubmed No.:27060717 Arctigenin but not arctiin acts as the major effective constituent of Arctium lappa L. fruit for attenuating colonic inflammatory response induced by dextran sulfate sodium in mice. (Application: Mouse WB) PMCID:    Pubmed No.:25284342 Interferon Regulatory Factor 7 Protects Against Vascular Smooth Muscle Cell Proliferation and Neointima Formation. PMCID:    Pubmed No.:25304854 Rhizoma Dioscoreae Nipponicae polysaccharides protect HUVECs from H2O2-induced injury by regulating PPARγ factor and the NADPH oxidase/ROS-NF-κB signal pathway. PMCID:    Pubmed No.:25305479 Norisoboldine ameliorates DSS-induced ulcerative colitis in mice through induction of regulatory T cells in colons. PMCID:    Pubmed No.:26363976 DGAEE, a newly synthesized derivative of glycyrrhetinic acid, potently attenuates mouse septic shock via its main metabolite DGA in an IL-10-dependent manner PMCID:    Pubmed No.:26456500 Apigenin and naringenin regulate glucose and lipid metabolism, and ameliorate vascular dysfunction in type 2 diabetic rats PMCID:    Pubmed No.:26801071 The Upregulation of TRAF1 Induced by Helicobacter pylori Plays an Antiapoptotic Effect on the Infected Cells PMCID:    Pubmed No.:27060717 Activation of Mesenchymal Stem Cells by Macrophages Prompts Human Gastric Cancer Growth through NF-κB Pathway PMCID:    Pubmed No.:24824968 Interferon Regulatory Factor 8 Protects Against Cerebral Ischaemic‐Reperfusion Injury PMCID:    Pubmed No.:24528256 Tris (2-chloroethyl) phosphate induces senescence-like phenotype of hepatocytes via the p21Waf1/Cip1-Rb pathway in a p53-independent manner PMCID:    Pubmed No.:28886428 Apigenin and naringenin regulate glucose and lipid metabolism, and ameliorate vascular dysfunction in type 2 diabetic rats PMCID:    Pubmed No.:26801071 Modified Si-Miao-San inhibits inflammation and promotes glucose disposal in adipocytes through regulation of AMP-kinase PMCID:    Pubmed No.:25556062 Astragaloside IV attenuates cognitive impairments induced by transient cerebral ischemia and reperfusion in mice via anti-inflammatory mechanisms PMCID:    Pubmed No.:28011393 The Role of TLR4 on PGC-1α-Mediated Oxidative Stress in Tubular Cell in Diabetic Kidney Disease PMCID:    Pubmed No.:29861832 Perilla aldehyde attenuates CUMS-induced depressive-like behaviors via regulating TXNIP/TRX/NLRP3 pathway in rats PMCID:    Pubmed No.:29800538 Phenyllactic acid promotes cell migration and invasion in cervical cancer via IKK/NF-κB-mediated MMP-9 activation PMCID:    Pubmed No.:31572058 IL-4 mediates the delayed neurobehavioral impairments induced by neonatal hepatitis B vaccination that involves the down-regulation of the IL-4 receptor in the hippocampus PMCID:    Pubmed No.:29751176 Luteolin Attenuates APEC-Induced Oxidative Stress and Inflammation via Inhibiting the HMGB1/TLR4/NF-κB Signal Axis in the Ileum of Chicks PMCID:    Pubmed No.:36611692
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
COPYRIGHT © 2015-2018 Bioworld Technology, Inc. All rights reserved POLYCLONAL AND MONOCLONAL ANTIBODY CENTER