ERK3 (phospho-S189) polyclonal antibody 
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Product Name :
ERK3 (phospho-S189) polyclonal antibody Background :
Mitogen-activated protein kinase (MAPK) signaling pathways involve closely related MAP kinases, including extracellular-signal-related kinase 3 (ERK 3, also designated PRKM6 and p97MAPK). Serum, growth factors and phorbol esters can initiate ERK 3 signaling pathways. Despite lacking a definitive nuclear localization sequence, ERK 3 constitutively localizes to the nucleus upon activation. p38 pathway activation-dependent upregulation of ERK 3 is independent of the status of p53, Bcl2 and caspase 3 during cell stress and damage induced by proteasome inhibition, suggesting ERK 3 in part mediates intracellular defense or cell rescue. The human ERK3 gene maps to chromosome 15q21 and encodes a 721 amino acid protein. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
P-ERK3(S189) polyclonal antibody detects endogenous levels of ERK3 protein only when phosphorylated at Ser189. Immunogen :
Synthetic phosphopeptide derived from human ERK3 around the phosphorylation site of Serine 189. Conjugate :
Unconjugated Modification :
Phosphorylation
ERK3 (phospho-S189) polyclonal antibody Background :
Mitogen-activated protein kinase (MAPK) signaling pathways involve closely related MAP kinases, including extracellular-signal-related kinase 3 (ERK 3, also designated PRKM6 and p97MAPK). Serum, growth factors and phorbol esters can initiate ERK 3 signaling pathways. Despite lacking a definitive nuclear localization sequence, ERK 3 constitutively localizes to the nucleus upon activation. p38 pathway activation-dependent upregulation of ERK 3 is independent of the status of p53, Bcl2 and caspase 3 during cell stress and damage induced by proteasome inhibition, suggesting ERK 3 in part mediates intracellular defense or cell rescue. The human ERK3 gene maps to chromosome 15q21 and encodes a 721 amino acid protein. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
P-ERK3(S189) polyclonal antibody detects endogenous levels of ERK3 protein only when phosphorylated at Ser189. Immunogen :
Synthetic phosphopeptide derived from human ERK3 around the phosphorylation site of Serine 189. Conjugate :
Unconjugated Modification :
Phosphorylation
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Western blot analysis of extracts of 293 cells, using Phospho-ERK3-S189 antibody.
Mineral trioxide aggregate upregulates odonto/osteogenic capacity of bone marrow stromal cells from craniofacial bones via JNK and ERK MAPK signalling pathways
PMCID: Pubmed No.:24635197
Mineral trioxide aggregate upregulates odonto/osteogenic capacity of bone marrow stromal cells from craniofacial bones via JNK and ERK MAPK signalling pathways
PMCID: Pubmed No.:24635197
Up-Regulated MicroRNA499a by Hepatitis B Virus Induced Hepatocellular Carcinogenesis via Targeting MAPK6
PMCID: Pubmed No.:25340781
An Integrated Proteomics and Bioinformatics Approach Reveals the Anti-inflammatory Mechanism of Carnosic Acid
PMCID: Pubmed No.:29713284
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.