DIAPH1 polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
DIAPH1 polyclonal antibody Background :
Dia 1 (also designated p140mDia) and Dia 2 (also designated p134mDia 2), mammalian homologs of Drosophila Diaphanous, belong to a family of formin-homology (FH) proteins, which are characterized by having tandemly aligned FH1 and FH2 domains in their carboxy terminal regions. The amino terminus of inactivated Dia 1 binds to the FH1 and FH2 domains, and is activated when GTP-bound Rho disrupts the intramolecular interaction of Dia 1 and converts it to an open conformation. Once activated, Dia 1 co-localizes with profilin and RhoA in dynamic plasma-membrane structures, and induces the formation of stress fibers in conjunction with the ROCK mediated signaling pathway. However, Dia 1 and ROCK activities antagonize each other in order to determine the thickness and density of the stress fibers formed. Dia 1 has also been implicated as the gene responsible for non-syndromic deafness. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
DIAPH1 polyclonal antibody detects endogenous levels of DIAPH1 protein. Immunogen :
Recombinant full length Human DIAPH1. Conjugate :
Unconjugated Modification :
Unmodification
DIAPH1 polyclonal antibody Background :
Dia 1 (also designated p140mDia) and Dia 2 (also designated p134mDia 2), mammalian homologs of Drosophila Diaphanous, belong to a family of formin-homology (FH) proteins, which are characterized by having tandemly aligned FH1 and FH2 domains in their carboxy terminal regions. The amino terminus of inactivated Dia 1 binds to the FH1 and FH2 domains, and is activated when GTP-bound Rho disrupts the intramolecular interaction of Dia 1 and converts it to an open conformation. Once activated, Dia 1 co-localizes with profilin and RhoA in dynamic plasma-membrane structures, and induces the formation of stress fibers in conjunction with the ROCK mediated signaling pathway. However, Dia 1 and ROCK activities antagonize each other in order to determine the thickness and density of the stress fibers formed. Dia 1 has also been implicated as the gene responsible for non-syndromic deafness. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
DIAPH1 polyclonal antibody detects endogenous levels of DIAPH1 protein. Immunogen :
Recombinant full length Human DIAPH1. Conjugate :
Unconjugated Modification :
Unmodification
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WesternBlot (WB) analysis of DIAPH1 polyclonal antibody
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.