ERI1 (D298) polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
ERI1 (D298) polyclonal antibody Background :
Helicase with RNase motif, more commonly designated Dicer, cleaves doublestranded RNA (dsRNA) in the RNA interference and small temporal RNA (stRNA) pathways, producing active small RNA components (siRNAs) which target the destruction of RNA and repress gene expression. Human Dicer cleaves dsRNA independent of ATP. The 3’-5’ exonuclease ERI-1, also known as Protein 3’hExo, degrades Histone mRNA after replication and may be involved in the regulation of RNA interference. ERI-1 has a high affinity for the stem-loop structure of replication-dependent Histone pre-mRNAs. It requires the 5’-ACCCA-3’ sequence present in stem-loop structure. ERI-1 and a stemloop binding protein (SLBP) target opposite faces of a unique highly conserved stem-loop RNA scaffold towards the 3’ end of Histone mRNA. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
ERI1 (D298) polyclonal antibody detects endogenous levels of ERI1 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 266-318 of Human ERI1. Conjugate :
Unconjugated Modification :
Unmodification
ERI1 (D298) polyclonal antibody Background :
Helicase with RNase motif, more commonly designated Dicer, cleaves doublestranded RNA (dsRNA) in the RNA interference and small temporal RNA (stRNA) pathways, producing active small RNA components (siRNAs) which target the destruction of RNA and repress gene expression. Human Dicer cleaves dsRNA independent of ATP. The 3’-5’ exonuclease ERI-1, also known as Protein 3’hExo, degrades Histone mRNA after replication and may be involved in the regulation of RNA interference. ERI-1 has a high affinity for the stem-loop structure of replication-dependent Histone pre-mRNAs. It requires the 5’-ACCCA-3’ sequence present in stem-loop structure. ERI-1 and a stemloop binding protein (SLBP) target opposite faces of a unique highly conserved stem-loop RNA scaffold towards the 3’ end of Histone mRNA. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
ERI1 (D298) polyclonal antibody detects endogenous levels of ERI1 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 266-318 of Human ERI1. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of ERI1 pAb at 1:500 dilution Lane1:SGC7901 whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:U-87MG whole cell lysate(40ug)
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.