Hairless (E77) polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
Hairless (E77) polyclonal antibody Background :
Drosophila genes can be categorized based on the type of protein for which they encode and are represented by six major classifications, which include intracellular signaling proteins, transmembrane proteins, RNA binding proteins, secreted factors, transcription regulators (basic helix-loop-helix, homeodomain containing, zinc finger containing and chromatin associated) or other functional proteins. Among these numerous proteins, Hairless (CG5460, CT36411, CT17282) is a transcriptional co-repressor that contains a “Paired box” domain, a KX-repeat domain, a NEDL-repeat domain and a serine-rich domain. Hairless is an antagonist of the Notch signaling pathway in Drosophila. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Hairless (E77) polyclonal antibody detects endogenous levels of Hairless protein. Immunogen :
Synthetic peptide, corresponding to amino acids 51-100 of Human Hairless. Conjugate :
Unconjugated Modification :
Unmodification
Hairless (E77) polyclonal antibody Background :
Drosophila genes can be categorized based on the type of protein for which they encode and are represented by six major classifications, which include intracellular signaling proteins, transmembrane proteins, RNA binding proteins, secreted factors, transcription regulators (basic helix-loop-helix, homeodomain containing, zinc finger containing and chromatin associated) or other functional proteins. Among these numerous proteins, Hairless (CG5460, CT36411, CT17282) is a transcriptional co-repressor that contains a “Paired box” domain, a KX-repeat domain, a NEDL-repeat domain and a serine-rich domain. Hairless is an antagonist of the Notch signaling pathway in Drosophila. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Hairless (E77) polyclonal antibody detects endogenous levels of Hairless protein. Immunogen :
Synthetic peptide, corresponding to amino acids 51-100 of Human Hairless. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of HR pAb at 1:500 dilution Lane1:The Small intestine tissue lysate of Rat(40ug) Lane2:A375 whole cell lysate(40ug) -
Immunohistochemistry (IHC) analyzes of Hairless (E77) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.