DDDDK-tag (1A8) monoclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
DDDDK-tag (1A8) monoclonal antibody Background :
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties. Product :
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
DDDDK-tag (1A8) mAb detects over-expressed or recombinant proteins containing the DDDDK epitope tag. Immunogen :
Synthetic peptide DDDDK-tag. Conjugate :
Unconjugated Modification :
Unmodification
DDDDK-tag (1A8) monoclonal antibody Background :
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties. Product :
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
DDDDK-tag (1A8) mAb detects over-expressed or recombinant proteins containing the DDDDK epitope tag. Immunogen :
Synthetic peptide DDDDK-tag. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of DDDDK-tag (1A8) mAb at 1:10000/1:20000/1:30000 dillution Lane1:HEK293T whole cell lysate ,untransfected Lane2:HEK293T whole cell lysate , transfected with pcDNA3.1-HA-DDDDK-p62 -
Western blot (WB) analysis of DDDDK-tag (1A8) mAb at 1:5000 dilution Lane1:HEK293T whole cell lysate ,untransfected Lane2:HEK293T whole cell lysate , transfected with pcDNA3.1-HA-DDDDK-p62 (Anti-SQSTM1, IP) Lane3:HEK293T whole cell lysate , transfected with pcDNA3.1-HA-DDDDK-p62 (Anti-DDDDK, IP) Lane4:HEK293T whole cell lysate , transfected with pcDNA3.1-HA-DDDDK-p62 (Anti-DDDDK, IP, supernatant) -
Western blot (WB) analysis of DDDDK-tag (1A8) mAb at 1:5000 dilution Lane1:HEK293T whole cell lysate ,untransfected Lane2:HEK293T whole cell lysate , transfected with pcDNA3.1-HA-DDDDK-p62 (Anti-SQSTM1, IP) Lane3:HEK293T whole cell lysate , transfected with pcDNA3.1-HA-DDDDK-p62 (Anti-DDDDK, IP) Lane4:HEK293T whole cell lysate , transfected with pcDNA3.1-HA-DDDDK-p62 (Anti-DDDDK, IP, supernatant)
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.