NLRP3 polyclonal antibody 
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Product Name :
NLRP3 polyclonal antibody Background :
Cryopyrin interacts selectively with apoptosis-associated specklike protein containing a CARD domain (ASC). This complex may function as an upstream activator of NFkB signaling and caspase-1 activation. The complex also inhibits TNFa induced activation and nuclear translocation of RelA/NFkB p65. Mutations in Cryopyrin and Pyrin proteins are responsible for several autoinflammatory disorders in humans, including familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS) and chronic infantile neurologic cutaneous and articular syndrome (CINCA). Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. Specificity :
NLRP3 polyclonal antibody detects endogenous levels of NLRP3 protein. Immunogen :
recombinant protein Conjugate :
Unconjugated Modification :
Unmodification
NLRP3 polyclonal antibody Background :
Cryopyrin interacts selectively with apoptosis-associated specklike protein containing a CARD domain (ASC). This complex may function as an upstream activator of NFkB signaling and caspase-1 activation. The complex also inhibits TNFa induced activation and nuclear translocation of RelA/NFkB p65. Mutations in Cryopyrin and Pyrin proteins are responsible for several autoinflammatory disorders in humans, including familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS) and chronic infantile neurologic cutaneous and articular syndrome (CINCA). Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. Specificity :
NLRP3 polyclonal antibody detects endogenous levels of NLRP3 protein. Immunogen :
recombinant protein Conjugate :
Unconjugated Modification :
Unmodification
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Western blot analysis of NLRP3 on human lung tissue lysates. -
ICC staining NLRP3 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. -
ICC staining NLRP3 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
DHA/AA alleviates LPS-induced Kupffer cells pyroptosis via GPR120 interaction with NLRP3 to inhibit inflammasome complexes assembly
PMCID: Pubmed No.:33436541
Clemastine improves hypomyelination in rats with hypoxic–ischemic brain injury by reducing microglia-derived IL-1β via P38 signaling pathway
PMCID: Pubmed No.:32061255
Paeoniflorin alleviates CFA-induced inflammatory pain by inhibiting TRPV1 and succinate/SUCNR1-HIF-1α/NLPR3 pathway
PMCID: Pubmed No.:34844873
Ginsenoside Rg1 ameliorates glomerular fibrosis during kidney aging by inhibiting NOX4 and NLRP3 inflammasome activation in SAMP8 mice
PMCID: Pubmed No.:32114413
Ginsenoside Rg1 ameliorates aging‑induced liver fibrosis by inhibiting the NOX4/NLRP3 inflammasome in SAMP8 mice
PMCID: Pubmed No.:34523690
ManNAc protects against podocyte pyroptosis via inhibiting mitochondrial damage and ROS/NLRP3 signaling pathway in diabetic kidney injury model
PMCID: Pubmed No.:35338958
Ginsenoside Rg1 attenuates LPS-induced chronic renal injury by inhibiting NOX4-NLRP3 signaling in mice
PMCID: Pubmed No.:35421784
Gondoic acid alleviates LPS‑induced Kupffer cells inflammation by inhibiting ROS production and PKCθ/ERK/STAT3 signaling pathway
PMCID: Pubmed No.:35998508
Dehydroepiandrosterone protects against oleic acid-triggered mitochondrial dysfunction to relieve oxidative stress and inflammation via activation of the AMPK-Nrf2 axis by targeting GPR30 in hepatocytes
PMCID: Pubmed No.:36773597
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.