CD31/PECAM-1 (T709) polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
CD31/PECAM-1 (T709) polyclonal antibody Background :
CD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells and endothelial cells, and modulates cell adhesion, endothelial cell migration and angiogenesis. CD31 is phosphorylated on Tyr686 at the cytoplasmic carboxy-terminal tail upon various stimuli (e.g. mechanical stress or oxidative stress), presumably by Src family members. The tyrosine phosphorylation mediates associations with a number of SH2 domain containing binding partners such as PI3-kinase, SHIP, PLCγ and SHP-2. Thus, CD31 serves as a scaffold for various signaling molecules. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
CD31/PECAM-1 (T709) polyclonal antibody detects endogenous levels of CD31 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 670-720 of Human CD31. Conjugate :
Unconjugated Modification :
Unmodification
CD31/PECAM-1 (T709) polyclonal antibody Background :
CD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells and endothelial cells, and modulates cell adhesion, endothelial cell migration and angiogenesis. CD31 is phosphorylated on Tyr686 at the cytoplasmic carboxy-terminal tail upon various stimuli (e.g. mechanical stress or oxidative stress), presumably by Src family members. The tyrosine phosphorylation mediates associations with a number of SH2 domain containing binding partners such as PI3-kinase, SHIP, PLCγ and SHP-2. Thus, CD31 serves as a scaffold for various signaling molecules. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
CD31/PECAM-1 (T709) polyclonal antibody detects endogenous levels of CD31 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 670-720 of Human CD31. Conjugate :
Unconjugated Modification :
Unmodification
-
Western blot (WB) analysis of CD31/PECAM-1 (T709) pAb at 1:500 dilution Lane1:A549 whole cell lysate(30ug) Lane2:Raw264.7 whole cell lysate(40ug) Lane3:C6 whole cell lysate(40ug) -
Immunohistochemistry (IHC) analyzes of CD31/PECAM-1 (T709) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
The regulatory mechanism of Hsp90a secretion from endothelial cells and its role in angiogenesis during wound healing
PMCID: Pubmed No.:20558142
Endostatin inhibits tumour lymphangiogenesis and lymphatic metastasis via cell surface nucleolin on lymphangiogenic endothelial cells
PMCID: Pubmed No.:20814900
Construction of a Portal Implantable Functional Tissue-Engineered Liver Using Perfusion-Decellularized Matrix and Hepatocytes in Rats
PMCID: Pubmed No.:21054928
Presence of glioma stroma mesenchymal stem cells in a murine orthotopic glioma model
PMCID: Pubmed No.:21298274
Silencing of hHS6ST2 inhibits progression of pancreatic cancer through inhibition of Notch signaling
PMCID: Pubmed No.:21443520
STAT3 Knockdown Reduces Pancreatic Cancer Cell Invasiveness and Matrix Metalloproteinase-7 Expression in Nude Mice
PMCID: Pubmed No.:21991388
MicroRNA-214 downregulation contributes to tumor angiogenesis via inducing secretion of hepatoma-derived growth factor in human hepatoma
PMCID: Pubmed No.:22613005
Inhibition of autophagy enhances anticancer effects of bevacizumab in hepatocarcinoma
PMCID: Pubmed No.:23052483
HIF-1α and HIF-2α: Siblings in Promoting Angiogenesis of Residual Hepatocellular Carcinoma after High-Intensity Focused Ultrasound Ablation
PMCID: Pubmed No.:24551189
Protein Tyrosine Phosphatase U (PTPRU) is Required for Glioma Growth and Motility
PMCID: Pubmed No.:24876153
Downregulation of FoxM1 inhibits proliferation, invasion and angiogenesis of HeLa cells in vitro and in vivo
PMCID: Pubmed No.:25201015
C3a Receptor Antagonist Ameliorates Inflammatory and Fibrotic Signals in Type 2 Diabetic Nephropathy by Suppressing the Activation of TGF-β/smad3 and IKBα Pathway.
PMCID: Pubmed No.:25422985
C3a and C5a Receptor Antagonists Ameliorate Endothelial-myofibroblast Transition via the Wnt/β-catenin Signaling Pathway in Diabetic Kidney Disease
PMCID: Pubmed No.:25682062
CXCL1 promotes tumor growth through VEGF pathway activation and is associated with inferior survival in gastric cancer
PMCID: Pubmed No.:25641338
An HCG-rich microenvironment contributes to ovarian cancer cell differentiation into endothelioid cells in a three-dimensional culture system
PMCID: Pubmed No.:26479853
HSPA12B attenuates acute lung injury during endotoxemia in mice
PMCID: Pubmed No.:26428851
The protective effect of melatonin on smoke‐induced vascular injury in rats and humans: a randomized controlled trial (RCT)
PMCID: Pubmed No.:26681403
Downregulation of FoxM1 inhibits proliferation, invasion and angiogenesis of HeLa cells in vitro and in vivo
PMCID: Pubmed No.:25201015
C3a Receptor Antagonist Ameliorates Inflammatory and Fibrotic Signals in Type 2 Diabetic Nephropathy by Suppressing the Activation of TGF-β/smad3 and IKBα Pathway.
PMCID: Pubmed No.:25422985
An HCG-rich microenvironment contributes to ovarian cancer cell differentiation into endothelioid cells in a three-dimensional culture system
PMCID: Pubmed No.:26479853
HSPA12B attenuates acute lung injury during endotoxemia in mice
PMCID: Pubmed No.:26428851
The protective effect of melatonin on smoke‐induced vascular injury in rats and humans: a randomized controlled trial (RCT)
PMCID: Pubmed No.:26681403
HIF-1α and HIF-2α: Siblings in Promoting Angiogenesis of Residual Hepatocellular Carcinoma after High-Intensity Focused Ultrasound Ablation
PMCID: Pubmed No.:24551189
Heat shock protein 60 stimulates the migration of vascular smooth muscle cells via Toll-like receptor 4 and ERK MAPK activation
PMCID: Pubmed No.:26477505
Overexpression and correlation of HIF-2α, VEGFA and EphA2 in residual hepatocellular carcinoma following high-intensity focused ultrasound treatment: Implications for tumor recurrence and progression
PMCID: Pubmed No.:28587437
CXCR1/2 antagonism with CXCL8/Interleukin-8 analogue CXCL8(3–72)K11R/G31P restricts lung cancer growth by inhibiting tumor cell proliferation and suppressing angiogenesis
PMCID: Pubmed No.:26087179
Growth of Human Colon Cancer Cells in Nude Mice is Delayed by Ketogenic Diet With or Without Omega-3 Fatty Acids and Medium-chain Triglycerides
PMCID: Pubmed No.:25773851
Resveratrol improves human umbilical cord-derived mesenchymal stem cells repair for cisplatin-induced acute kidney injury
PMCID: Pubmed No.:30237401
Anti-tumor effect of local injectable hydrogel-loaded endostatin alone and in combination with radiotherapy for lung cancer
PMCID: Pubmed No.:33427520
Enhanced Anti-Cancer Effect of Folate-Conjugated Olaparib Nanoparticles Combined with Radiotherapy in Cervical Carcinoma
PMCID: Pubmed No.:33328733
Preparation, characterization, in vitro and in vivo anti-tumor effect of thalidomide nanoparticles on lung cancer
PMCID: Pubmed No.:29719394
Human three-dimensional dental pulp organoid model for toxicity screening of dental materials on dental pulp cells and tissue
PMCID: Pubmed No.:34587308
Downregulating Heparanase-Induced Vascular Normalization: A New Approach To Increase the Bioavailability of Chemotherapeutics in Solid Tumors
PMCID: Pubmed No.:30059227
Oridonin inhibits tumor angiogenesis and induces vessel normalization in experimental colon cancer
PMCID: Pubmed No.:33976735
Erteng-Sanjie Capsule Enhances Chemosensitivity of 5-Fluorouracil in Tumor-Bearing Nude Mice with Gastric Cancer by Inhibiting Notch1/Hes1 Signaling Pathway
PMCID: Pubmed No.:34257696
Brown adipose tissue involution associated with progressive restriction in progenitor competence
PMCID: Pubmed No.:35417710
JAK1/2 Inhibitor Baricitinib Improves Skin Fibrosis and Digital Ulcers in Systemic Sclerosis
PMCID: Pubmed No.:35733864
Inhibition of TRPV4 remodels single cell polarity and suppresses the metastasis of hepatocellular carcinoma
PMCID: Pubmed No.:37369706
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.