Product Name :
ChemR23 (R249) polyclonal antibody Background :
Chemokine receptor-like 1 (also designated G protein-coupled receptor DEZ or ChemR23) belongs to the G protein-coupled receptor 1 family. It is an integral membrane protein functioning as a receptor, possibly a chemotactic peptide receptor. It also acts as a co-receptor for various SIV strains and for a primary HIV-1 strain. ChemR23 is highly expressed in developing osseous and cartilaginous tissue, brain, kidney, gastrointestinal tissues and myeloid tissue, as well as in adult parathyroid glands. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
ChemR23 (R249) polyclonal antibody detects endogenous levels of ChemR23 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 216-265 of Human MChemR23. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of CMKLR1 pAb at 1:500 dilution Lane1:RAW264.7 whole cell lysate(40ug) Lane2:PMVEC whole cell lysate(40ug) Lane3:HUT78 whole cell lysate(40ug) Lane4:A549 whole cell lysate(40ug) Lane5:LO2 whole cell lysate(40ug)
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  Immunohistochemistry (IHC) analyzes of ChemR23 (R249) pAb in paraffin-embedded human kidney carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
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  Immunohistochemistry (IHC) analyzes of ChemR23 (R249) pAb in paraffin-embedded human kidney carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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