Product Name :
Goat Anti-Rabbit IgG (H+L) HRP Background :
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rabbit IgG.It also reacts with the light chains of other rabbit immunoglobuins.NO antibody was detected against non immunoglobulin serum proteins.The antibody may cross-react with immunoglobulins from other species. Product :
1 mg/ml in 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6,containing 1% BSA Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Immunogen :
Conjugate :
HRP Modification :
Unmodification
Goat Anti-Rabbit IgG (H+L) HRP Background :
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rabbit IgG.It also reacts with the light chains of other rabbit immunoglobuins.NO antibody was detected against non immunoglobulin serum proteins.The antibody may cross-react with immunoglobulins from other species. Product :
1 mg/ml in 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6,containing 1% BSA Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Immunogen :
Conjugate :
HRP Modification :
Unmodification
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Western blot (WB) analysis of β-actin polyclonal antibody at 1:5000 dilution,Goat Anti-Rabbit IgG (H+L) HRP at 1:5000,1:10000,1:20000 dilution Lane1:SGC7901 whole cell lysate(20ug) Lane2:A5491 whole cell lysate(20ug) Lane3:HEK293T whole cell lysate(20ug)
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.