Product Name :
SEMA4C polyclonal antibody Background :
SEMA4C (semaphorin-4C), also known as SEMAF, is an 833 amino acid single-pass type I membrane protein that contains one sema domain, one PSI domain and one Ig-like C2-type domain. Expressed in a variety of tissues, including lung, kidney and brain, SEMA4C interacts with GIPC and NCDN and is thought to play a role in the formation of neural networks during development. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
SEMA4C polyclonal antibody detects endogenous levels of SEMA4C protein. Immunogen :
Synthetic peptide, corresponding to Human SEMA4C. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of SEMA4C polyclonal antibody at 1:500 dilution LaneA:A549 whole cell lysate(40ug) LaneB:The mouse brain lysate of Mouse(40ug) LaneC:The rat brain lysate of Mouse(40ug)

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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