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PPP1R14D polyclonal antibody BS62437
  • Western blot analysis of extracts of human kidney line, using PPP1R14D polyclonal antibody.
Product NamePPP1R14D polyclonal antibody
Catalog No.BS62437
Swiss-ProtQ9NXH3
Host Rabbit
ReactivityHuman
ApplicationsWB IHC
Application_allWB:1:500~1:1000 IHC:1:50~1:200
BiowMW~ 24 kDa
Alternative NameProtein phosphatase 1 regulatory subunit 14D; Gastrointestinal and brain-specific PP1-inhibitory protein 1; GBPI-1; PPP1R14D; GBPI
Purification&PurityThe protein was purified from E.coli and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50ul $158
100ul $275
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Product Name :
PPP1R14D polyclonal antibody
Background :
Protein phosphatase 1 regulatory subunit 14D, inhibitor of PPP1CA. Has inhibitory activity only when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4.
Storage&Stability :
Store at 4°C short term. Aliquot and store at -25°C long term. Avoid freeze-thaw cycles.
Specificity :
PPP1R14D polyclonal antibody detects endogenous levels of PPP1R14D protein.
Immunogen :
Synthetic peptide, corresponding to Human PPP1R14D.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot analysis of extracts of human kidney line, using PPP1R14D polyclonal antibody.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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