Product Name :
Septin 3 polyclonal antibody Background :
The septins are a family of GTPase enzymes, some of which are required for cytokinesis and others of which are associated with exocytosis. Members of the Septin family can form heteropolymer complexes and also play a role in the organization of new growth in organisms. The transcriptional regulation of all septins is complex, resulting in alternatively spliced variants. Septin 10, a 517 residue polypeptide which localizes to the cytoplasm and nucleus, shares closest homology to Septin 6 and Septin 8. Septin 10 is expressed ubiquitously, though most abundantly in the placenta, lung, kidney, heart, skeletal muscles, liver and various tumor cell lines. Like other Septin family members, Septin 10 displays GTP-binding and GTPase activity. Additionally, Septin 10 is potentially involved in cytokinesis. Upon maturation induced by lipopolysaccharide (LPS), dendritic cells express upregulated amounts of Septin 10. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Septin 3 polyclonal antibody detects endogenous levels of Septin 3 protein. Immunogen :
Synthetic peptide, corresponding to Human Septin 3. Conjugate :
Unconjugated Modification :
Unmodification

• 
  Western blot (WB) analysis of Septin 3 polyclonal antibody at 1:500 dilution LaneA:The brain tissue lysate of Mouse LaneB:The brain tissue lysate of Rat LaneC:Hela whole cell lysate LaneD:The kidney tissue lysate of Mouse LaneE:The kidney tissue lysate of Rat

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

---

Price/Size :

USD 368/1mg/vial

---

---

Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

---

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

---

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

---

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

---

Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

---