Product Name :
Mannose Binding Protein polyclonal antibody Background :
Mannose-binding lectin protein C (MBL-C), also known as mannose-binding protein C; mannose-binding lectin 2, soluble (opsonic defect); mannan-binding lectin; mannan-binding protein; and soluble mannose-binding lectin, initiates the lectin branch of the innate immune response by binding to the surface of potentially pathogenic microorganisms and initiating complement fixation through an N-terminal collagen-like domain. MBL-C is a key component in immune response due to its ability to directly trigger neutralization of invading microorganisms by an Ab-independent mechanism. It binds to sugars on the surface of bacterial, fungal and parasitic cells through C-terminal, Ca2+-dependent carbohydrate-recognition domains. Mutations of human MBL are associated with immunodeficiency resulting from a reduction in the ability of the mutant MBL to initiate complement fixation. In human, two types of MBL-associated serine proteases (MASP-1 and MASP-2) and a truncated form of MASP-2, designated small MBL-associated protein (sMAP) or MAp19, complex with MBL to activate the lectin pathway of the complement system. Activated MASPs subsequently cleave and activate downstream components of the complement pathway. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Mannose Binding Protein polyclonal antibody detects endogenous levels of Mannose Binding protein. Immunogen :
Synthetic peptide, corresponding to Human Mannose Binding Protein. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of Mannose Binding Protein polyclonal antibody at 1:500 dilution LaneA:HepG2 whole cell lysate LaneB:Aml-12 whole cell lysate LaneC:PC12 whole cell lysate LaneD:L02 whole cell lysate

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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