Product Name :
CD322 polyclonal antibody Background :
Junctional adhesion molecule (JAM) is a member of the immunoglobulin superfamily expressed in tight junctions of epithelial cells and endothelial cells. It is implicated in transendothelial migration of leukocytes. JAM is constitutively expressed on circulating monocytes, neutrophils, lymphocyte subsets and platelets. The JAM family consists of JAM-A, JAM-B and JAM-C, alternatively designated JAM-1, JAM-2 and JAM-3, respectively. JAM-A localizes with F-Actin at the cell-cell contacts and at the membrane ruffles. It is involved in cell to cell adhesion through homophilic interactions and plays a role in the organization of tight junctions and modulation of leukocyte extravasation. JAM-B interacts with discrete subsets of PBLs, suggesting that it may play a role in lymphocyte trafficking. JAM-B and JAM-C proteins are binding partners; JAM-C may be a functional JAM-B receptor. Specifically, JAM-B adheres to T cells through heterotypic interactions with JAM-C. The JAM-B/JAM-C interaction may play a role in T, NK and dendritic cellular inflammation. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
CD322 polyclonal antibody detects endogenous levels of CD322 protein. Immunogen :
Synthetic peptide, corresponding to Human CD322. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of CD322 polyclonal antibody at 1:500 dilution LaneA:A549 whole cell lysate LaneB:HEK293T whole cell lysate LaneC:MEF whole cell lysate

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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