Product Name :
RHCG polyclonal antibody Background :
RhCG (Rhesus blood group family type C glycoprotein), also known as RHGK (Rh glycoprotein kidney), PDRC2 (tumor-related protein DRC2) or SLC42A3, is a multi-pass membrane protein with three potential N-glycosylation sites that belongs to the Rh subfamily of the ammonium transporter family. Expressed in a wide variety of tissues with predominant expression in adult and fetal kidney, RhCG localizes to the apical and/or basolateral cell membrane (depending on the species) and is believed to function as a non-erythroid, bidirectional, electroneutral ammonium transporter. Specifically, RhCG is found throughout the kidney in sites such as the CNT (connecting segment), the DCT (distal convoluted tubule), the collecting duct and the ICT (initial collecting tubule), that are major contributors to renal ammonia secretion. This suggests that RhCG plays an important role in renal ammonia metabolism. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
RHCG polyclonal antibody detects endogenous levels of RHCG protein. Immunogen :
Synthetic peptide, corresponding to Human RHCG. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of RHCG polyclonal antibody at 1:500 dilution LaneA:A549 whole cell lysate LaneB:Panc1 whole cell lysate LaneC:MEF whole cell lysate

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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