Granuphilin polyclonal antibody 
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MSDS
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Product Name :
Granuphilin polyclonal antibody Background :
Granuphilin, also designated synaptotagmin-like protein 4 or exophilin 2, is a member of the synaptotagmin-like protein family. In rodents, two isoforms have been characterized as granuphlin-a and granuphilin-b, and alternative splicing in human has also been described. Granuphilin-b is a shorter isoform which contains a different C-terminal sequence than the longer isoform. Granuphilin is part of a ternary complex consisting of Syntaxin 1A (STX1A) and Rab 27A. The interaction between granuphilin and Syntaxin 1A on the plasma membrane is regulated by Rab 27A. Granuphilin is a peripheral membrane protein mainly expressed in pancreatic β-cells and in the pituitary gland. It has been detected on secretory granules and close to the plasma membrane. In the pancreas, granuphilin interacts with Insulin-containing vesicles while in both the pancreas and pituitary, granuphilin modulates the secretion of hormones and the exocytosis of dense-core granules. Overexpression of granuphilin enhances basal Insulin secretion but also inhibits high K+-induced Insulin secretion. The effect of granuphilin on Insulin secretion may be impaired by a mutation that disrupts the binding to either Rab 27A or Syntaxin 1A, making granuphilin a possible regulator in the exocytotic pathway Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Granuphilin polyclonal antibody detects endogenous levels of Granuphilin protein. Immunogen :
Synthetic peptide, corresponding to Human Granuphilin. Conjugate :
Unconjugated Modification :
Unmodification
Granuphilin polyclonal antibody Background :
Granuphilin, also designated synaptotagmin-like protein 4 or exophilin 2, is a member of the synaptotagmin-like protein family. In rodents, two isoforms have been characterized as granuphlin-a and granuphilin-b, and alternative splicing in human has also been described. Granuphilin-b is a shorter isoform which contains a different C-terminal sequence than the longer isoform. Granuphilin is part of a ternary complex consisting of Syntaxin 1A (STX1A) and Rab 27A. The interaction between granuphilin and Syntaxin 1A on the plasma membrane is regulated by Rab 27A. Granuphilin is a peripheral membrane protein mainly expressed in pancreatic β-cells and in the pituitary gland. It has been detected on secretory granules and close to the plasma membrane. In the pancreas, granuphilin interacts with Insulin-containing vesicles while in both the pancreas and pituitary, granuphilin modulates the secretion of hormones and the exocytosis of dense-core granules. Overexpression of granuphilin enhances basal Insulin secretion but also inhibits high K+-induced Insulin secretion. The effect of granuphilin on Insulin secretion may be impaired by a mutation that disrupts the binding to either Rab 27A or Syntaxin 1A, making granuphilin a possible regulator in the exocytotic pathway Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Granuphilin polyclonal antibody detects endogenous levels of Granuphilin protein. Immunogen :
Synthetic peptide, corresponding to Human Granuphilin. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of Granuphilin polyclonal antibody at 1:500 dilution LaneA:CT26 whole cell lysate
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.