Product Name :
CLIC4 polyclonal antibody Background :
CLIC4 (chloride intracellular channel 4), also known as H1, huH1, p64H1, CLIC4L or MTCLIC, is a 253 amino acid single-pass membrane protein that localizes to both nucleus and cytoplasm and contains one GST C-terminal domain. Expressed in placenta, heart and skeletal muscle, as well as in epithelial cells from kidney, colon and esophageal tissue, CLIC4 functions as a monomer that is able to form selective ion channels in target proteins, thereby facilitating the transport of chloride and other ions. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
CLIC4 polyclonal antibody detects endogenous levels of CLIC4 protein. Immunogen :
Synthetic peptide, corresponding to Human CLIC4. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of CLIC4 polyclonal antibody at 1:500 dilution LaneA:COS-7 whole cell lysate LaneB:LOVO whole cell lysate LaneC:CT26 whole cell lysate LaneD:PC12 whole cell lysate

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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