PINK1 (Phospho-S228) polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
PINK1 (Phospho-S228) polyclonal antibody Background :
PTEN induced putative kinase 1, PINK1, is a mitochondrial serine/threonine kinase involved in the normal function and integrity of mitochondria, as well as in reduction of cytochrome c release from mitochondria. PINK1 phosphorylates Parkin and promotes its translocation to mitochondria. Research studies have shown that mutations in PINK1 are linked to autosomal recessive early onset Parkinson’s disease, and are associated with loss of protective function, mitochondrial dysfunction, aggregation of α-synuclein, as well as proteasome dysfunction. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
PINK1 (Phospho-S228) polyclonal antibody detects endogenous levels of PINK1 protein only when phosphorylated at Ser228. Immunogen :
Synthetic phosphopeptide derived from human PINK1 around the phosphorylation site of Serine 228. Conjugate :
Unconjugated Modification :
Phosphorylation
PINK1 (Phospho-S228) polyclonal antibody Background :
PTEN induced putative kinase 1, PINK1, is a mitochondrial serine/threonine kinase involved in the normal function and integrity of mitochondria, as well as in reduction of cytochrome c release from mitochondria. PINK1 phosphorylates Parkin and promotes its translocation to mitochondria. Research studies have shown that mutations in PINK1 are linked to autosomal recessive early onset Parkinson’s disease, and are associated with loss of protective function, mitochondrial dysfunction, aggregation of α-synuclein, as well as proteasome dysfunction. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
PINK1 (Phospho-S228) polyclonal antibody detects endogenous levels of PINK1 protein only when phosphorylated at Ser228. Immunogen :
Synthetic phosphopeptide derived from human PINK1 around the phosphorylation site of Serine 228. Conjugate :
Unconjugated Modification :
Phosphorylation
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Western blot (WB) analysis of PINK1 (Phospho-S228) polyclonal antibody at 1:500 dilution LaneA:The Testis tissue lysate of Mouse LaneB:The Testis tissue lysate of Rat LaneC:HEK293T whole cell lysate LaneD:MCF-7 whole cell lysate LaneE:U-87MG whole cell lysate
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.