Kir6.2 (Phospho-T224) polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
Kir6.2 (Phospho-T224) polyclonal antibody Background :
ATP-sensitive K+ channels play important roles in many cellular functions by coupling cell metabolism to electrical activity. KIR6.1 and KIR6.2 are members of the KIR (inwardly rectifying potassium channel) family of potassium channels. Inward rectifying K+ channels possess a greater tendency to allow potasium to flow into the cell rather than out of it. These channels comprise two subunits: a KIR6.0 subfamily component and a SUR component, which is a member of the ATP-binding cassette protein superfamily. Mutations in the gene coding for these channels are a cause of an autosomal recessive disorder characterized by unregulated insulin secretion. The amino-terminal and carboxyl-terminal domains of KIR channel subunits are both intracellular, and the two intracellular domains of KIR6.2 physically interact with each other. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Kir6.2 (Phospho-T224) polyclonal antibody detects endogenous levels of Kir6.2 protein only when phosphorylated at Thr224. Immunogen :
Synthetic phosphopeptide derived from human Kir6.2 around the phosphorylation site of Threonine 224. Conjugate :
Unconjugated Modification :
Phosphorylation
Kir6.2 (Phospho-T224) polyclonal antibody Background :
ATP-sensitive K+ channels play important roles in many cellular functions by coupling cell metabolism to electrical activity. KIR6.1 and KIR6.2 are members of the KIR (inwardly rectifying potassium channel) family of potassium channels. Inward rectifying K+ channels possess a greater tendency to allow potasium to flow into the cell rather than out of it. These channels comprise two subunits: a KIR6.0 subfamily component and a SUR component, which is a member of the ATP-binding cassette protein superfamily. Mutations in the gene coding for these channels are a cause of an autosomal recessive disorder characterized by unregulated insulin secretion. The amino-terminal and carboxyl-terminal domains of KIR channel subunits are both intracellular, and the two intracellular domains of KIR6.2 physically interact with each other. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Kir6.2 (Phospho-T224) polyclonal antibody detects endogenous levels of Kir6.2 protein only when phosphorylated at Thr224. Immunogen :
Synthetic phosphopeptide derived from human Kir6.2 around the phosphorylation site of Threonine 224. Conjugate :
Unconjugated Modification :
Phosphorylation
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Western blot (WB) analysis of Kir6.2 (Phospho-T224) polyclonal antibody at 1:500 dilution LaneA:MCF-7 whole cell lysate LaneB:The Liver tissue lysate of Mouse LaneC:The Liver tissue lysate of Rat
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.