NET (Phospho-S357) polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
NET (Phospho-S357) polyclonal antibody Background :
Numerous cellular functions, such as proliferation, differentiation, apoptosis, vesicular trafficking, nuclear transport and cytoskeletal organization, are controlled by GTPases. It has become increasingly clear that GTPases act in cascades in which their activities are linked by GTPase-activating proteins (GAPs) and guanine nucleotide exchange factors (GEFs). Researchers looking for new epithelial cell-specific oncogenes, using a highly efficient cDNA expression cloning system, have isolated the Ost oncogene from rat osteosarcoma cells. The Ost proto-oncogene protein contains DH and PH domains, catalyzes guanine nucleotide exchange on Rho A and Cdc42, and interacts specifically with the GTP-bound form of Rac1. The related NET1 protein also contains a DH domain and is ubiquitously expressed in a variety of tissues. Overexpression of NET1 in NIH/3T3 cells results in altered growth properties and tumorigenesis when injected into nude mice. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
NET (Phospho-S357) polyclonal antibody detects endogenous levels of NET protein only when phosphorylated at Ser357. Immunogen :
Synthetic phosphopeptide derived from human NET around the phosphorylation site of Serine 357. Conjugate :
Unconjugated Modification :
Phosphorylation
NET (Phospho-S357) polyclonal antibody Background :
Numerous cellular functions, such as proliferation, differentiation, apoptosis, vesicular trafficking, nuclear transport and cytoskeletal organization, are controlled by GTPases. It has become increasingly clear that GTPases act in cascades in which their activities are linked by GTPase-activating proteins (GAPs) and guanine nucleotide exchange factors (GEFs). Researchers looking for new epithelial cell-specific oncogenes, using a highly efficient cDNA expression cloning system, have isolated the Ost oncogene from rat osteosarcoma cells. The Ost proto-oncogene protein contains DH and PH domains, catalyzes guanine nucleotide exchange on Rho A and Cdc42, and interacts specifically with the GTP-bound form of Rac1. The related NET1 protein also contains a DH domain and is ubiquitously expressed in a variety of tissues. Overexpression of NET1 in NIH/3T3 cells results in altered growth properties and tumorigenesis when injected into nude mice. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
NET (Phospho-S357) polyclonal antibody detects endogenous levels of NET protein only when phosphorylated at Ser357. Immunogen :
Synthetic phosphopeptide derived from human NET around the phosphorylation site of Serine 357. Conjugate :
Unconjugated Modification :
Phosphorylation
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Western blot (WB) analysis of NET (Phospho-S357) polyclonal antibody at 1:500 dilution LaneA:Zebrafish
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.