CDK7 (Phospho-T170) polyclonal antibody 
Datasheet
COA
MSDS
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Product Name :
CDK7 (Phospho-T170) polyclonal antibody Background :
Cdk7 is a member of the cyclin dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. Cdk7 forms a trimeric complex with cyclin H and MAT1, which functions as a Cdk activating kinase (CAK). It is an essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. This protein is thought to serve as a direct link between the regulation of transcription and the cell cycle. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
CDK7 (Phospho-T170) polyclonal antibody detects endogenous levels of CDK7 protein only when phosphorylated at Thr170. Immunogen :
Synthetic phosphopeptide derived from human CDK7 around the phosphorylation site of Threonine 170. Conjugate :
Unconjugated Modification :
Phosphorylation
CDK7 (Phospho-T170) polyclonal antibody Background :
Cdk7 is a member of the cyclin dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. Cdk7 forms a trimeric complex with cyclin H and MAT1, which functions as a Cdk activating kinase (CAK). It is an essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. This protein is thought to serve as a direct link between the regulation of transcription and the cell cycle. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
CDK7 (Phospho-T170) polyclonal antibody detects endogenous levels of CDK7 protein only when phosphorylated at Thr170. Immunogen :
Synthetic phosphopeptide derived from human CDK7 around the phosphorylation site of Threonine 170. Conjugate :
Unconjugated Modification :
Phosphorylation
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Western blot (WB) analysis of CDK7 (Phospho-T170) polyclonal antibody at 1:500 dilution LaneA:Hela whole cell lysate LaneB:HEK293T whole cell lysate LaneC:A549 whole cell lysate LaneD:MCF-7 whole cell lysate LaneE:Raw264.7 whole cell lysate LaneF:The Spleen tissue lysate of Mouse
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.