Product Name :
DBI polyclonal antibody Background :
Long chain acyl-CoA esters (LCAs) act as both substrates and intermediates in metabolism, and as regulators of various intracellular functions. Acyl-CoA binding protein (ACBP) specifically binds to LCA with high affinity and regulates its availability. ACBP is structurally and functionally conserved among a diverse group of organisms, including human, rat, frog, insect, plant and yeast. DBI, the gene encoding human ACBP, which maps to chromosome 2, is highly expressed in liver, soleus muscle and heart. The ACBP protein is also abundant in cells with a high level of lipogenesis and de novo fatty acid synthesis. Expression of ACBP is significantly induced during adipocyte differentiation. DBI is a target gene for proliferator-activated receptor (PPAR) γ, and is directly activated by PPARγ/RXR&aipha; and PPAR&aipha;/RXR&aipha;, but not by PPARδ/ RXR&aipha;. In addition to acyl-CoA binding and transport, ACBP is also implicated in g-aminobutyric acid type A receptor binding, steroidogenesis and peptide hormone release. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
DBI polyclonal antibody detects endogenous levels of DBI protein. Immunogen :
A synthetic peptide corresponding to residues in Human DBI. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of DBI pAb at 1:1000 dilution Lane1:PC12 whole cell lysate(40ug) Lane2:CT-26 whole cell lysate(40ug) Lane3:THP-1 whole cell lysate(40ug) Lane4:The liver tissue lysate of Mouse(40ug) Lane5:The heart tissue lysate of Rat(40ug) Lane6:MCF-7 whole cell lysate(40ug) Lane7:HepG2 whole cell lysate(40ug)

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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