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p-MAPK14(Y182) polyclonal antibody BS4766
  • Western blot (WB) analysis of p-MAPK14(Y182) pAb at 1:500 dilution Lane1:Panc1 whole cell lysate(40ug) Lane2:Raw264.7 whole cell lysate(40ug) Lane3:PC12 whole cell lysate(40ug)
Product Namep-MAPK14(Y182) polyclonal antibody
Catalog No.BS4766
Swiss-ProtQ16539
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB
Application_allWB: 1:500~1:1000
BiowMW~ 43 kDa
Alternative NameMitogen-activated protein kinase 14; MAP kinase 14; MAPK 14; Cytokine suppressive anti-inflammatory drug-binding protein; CSAID-binding protein; CSBP; MAP kinase MXI2; MAX-interacting protein 2; Mitogen-activated protein kinase p38 alpha; MAP kinase p38 alpha; Stress-activated protein kinase 2a; SAPK2a; MAPK14; CSBP; CSBP1; CSBP2; CSPB1; MXI2; SAPK2A
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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50ul $168
100ul $298
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Product Name :
p-MAPK14(Y182) polyclonal antibody
Background :
MAP (mitogen-activated protein) kinases play a significant role in many biological processes, including cell adhesion and spreading, cell differentiation and apoptosis. p38α, p38β and p38γ, also known as MAPK14, MAPK11 and MAPK12, respectively, each contain one protein kinase domain and belong to the MAP kinase family. Expressed in different areas throughout the body with common expression patterns in heart, p38 proteins use magnesium as a cofactor to catalyze the ATP-dependent phosphorylation of target proteins. Via their catalytic activity, p38α, p38β and p38γ are involved in a variety of events throughout the cell, including signal transduction pathways, cytokine production and cell proliferation and differentiation.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
p-MAPK14(Y182) polyclonal antibody detects endogenous levels of MAPK14(Y182) protein only when phosphorylated at Tyr182.
Immunogen :
Synthetic phosphopeptide derived from human p38 around the phosphorylation site of Tyrosine 182.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot (WB) analysis of p-MAPK14(Y182) pAb at 1:500 dilution Lane1:Panc1 whole cell lysate(40ug) Lane2:Raw264.7 whole cell lysate(40ug) Lane3:PC12 whole cell lysate(40ug)
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PMCID:    Pubmed No.:25105919 Arctigenin but not arctiin acts as the major effective constituent of Arctium lappa L. fruit for attenuating colonic inflammatory response induced by dextran sulfate sodium in mice. (Application: Mouse WB) PMCID:    Pubmed No.:25284342 Potent Effects of the Total Saponins from Dioscorea nipponica Makino Against Streptozotocin-Induced Type 2 Diabetes Mellitus in Rats. PMCID:    Pubmed No.:25322985 The protective effects of urocortin1 against intracerebral hemorrhage by activating JNK1/2 and p38 phosphorylation and further increasing VEGF via corticotropin-releasing factor receptor 2. PMCID:    Pubmed No.:25576701 Down-regulation of ATF2 in the inhibition of T-2-toxin-induced chondrocyte apoptosis by selenium chondroitin sulfate nanoparticles PMCID:    Pubmed No.:None An Apple Oligogalactan Potentiates the Growth Inhibitory Effect of Celecoxib on Colorectal Cancer PMCID:    Pubmed No.:24274457 Phloretin induces apoptosis of non-small cell lung carcinoma A549 cells via JNK1/2 and p38 MAPK pathways PMCID:    Pubmed No.:26503828 Norisoboldine ameliorates DSS-induced ulcerative colitis in mice through induction of regulatory T cells in colons. PMCID:    Pubmed No.:26363976 DGAEE, a newly synthesized derivative of glycyrrhetinic acid, potently attenuates mouse septic shock via its main metabolite DGA in an IL-10-dependent manner PMCID:    Pubmed No.:26456500 Dioscin suppresses human laryngeal cancer cells growth via induction of cell-cycle arrest and MAPK-mediated mitochondrial-derived apoptosis and inhibition of tumor invasion. PMCID:    Pubmed No.:26849940 Dexmedetomidine Inhibits Maturation and Function of Human Cord Blood-Derived Dendritic Cells by Interfering with Synthesis and Secretion of IL-12 and IL-23 PMCID:    Pubmed No.:27054340 Role of cathepsin B in regulating migration and invasion of fibroblast‐like synoviocytes into inflamed tissue from patients with rheumatoid arthritis PMCID:    Pubmed No.:24749816 Platelet-derived growth factor-BB induces matrix metalloproteinase-2 expression and rat vascular smooth muscle cell migration via ROCK and ERK/p38 MAPK pathways. PMCID:    Pubmed No.:24792035 Role of p38, ERK1/2, focal adhesion kinase, RhoA/ROCK and cytoskeleton in the adipogenesis of human mesenchymal stem cells. PMCID:    Pubmed No.:2433197 Rho kinase inhibition by fasudil suppresses lipopolysaccharide-induced apoptosis of rat pulmonary microvascular endothelial cells via JNK and p38 MAPK pathway PMCID:    Pubmed No.:24406296 Protective effects of the total saponins from Dioscorea nipponica Makino against carbon tetrachloride-induced liver injury in mice through suppression of apoptosis and inflammation PMCID:    Pubmed No.:24491258 The DPP-4 inhibitor sitagliptin attenuates the progress of atherosclerosis in apolipoprotein-E-knockout mice via AMPK-and MAPK-dependent mechanisms PMCID:    Pubmed No.:24490809 Dentin matrix proteins (DMPs) enhance differentiation of BMMSCs via ERK and P38 MAPK pathways PMCID:    Pubmed No.:24562313 ZnO nanoparticle-induced oxidative stress triggers apoptosis by activating JNK signaling pathway in cultured primary astrocytes PMCID:    Pubmed No.:24624962 Mineral trioxide aggregate upregulates odonto/osteogenic capacity of bone marrow stromal cells from craniofacial bones via JNK and ERK MAPK signalling pathways PMCID:    Pubmed No.:24635197 Long-term blue light exposure induces RGC-5 cell death in vitro: involvement of mitochondria-dependent apoptosis, oxidative stress, and MAPK signaling pathways PMCID:    Pubmed No.:24682673 Tris (2-chloroethyl) phosphate induces senescence-like phenotype of hepatocytes via the p21Waf1/Cip1-Rb pathway in a p53-independent manner PMCID:    Pubmed No.:28886428 Combined treatment with vitamin C and methotrexate inhibits triple-negative breast cancer cell growth by increasing H2O2 accumulation and activating caspase-3 and p38 pathways PMCID:    Pubmed No.:28259996 Regulation of RAW 264.7 cell-mediated immunity by polysaccharides from Agaricus blazei Murill via the MAPK signal transduction pathway PMCID:    Pubmed No.:28322382 Pretreatment with low dose etomidate prevents etomidate-induced rat adrenal insufficiency by regulating oxidative stress-related MAPKs and apoptosis PMCID:    Pubmed No.:25989534 Dexmedetomidine Inhibits Maturation and Function of Human Cord Blood-Derived Dendritic Cells by Interfering with Synthesis and Secretion of IL-12 and IL-23 PMCID:    Pubmed No.:27054340 Downregulation of Foxc2 enhances apoptosis induced by 5-fluorouracil through activation of MAPK and AKT pathways in colorectal cancer PMCID:    Pubmed No.:26893778 Combined treatment with vitamin E and gefitinib has synergistic effects to inhibit TGF-β1-induced renal fibroblast proliferation PMCID:    Pubmed No.:27109695 A phycocyanin derived eicosapeptide attenuates lung fibrosis development PMCID:    Pubmed No.:34280398 Ochratoxin A-Triggered Chicken Heterophil Extracellular Traps Release through Reactive Oxygen Species Production Dependent on Activation of NADPH Oxidase, ERK, and p38 MAPK Signaling Pathways PMCID:    Pubmed No.:31523955 Anti-Cancer Effects of Sulfasalazine and Vitamin E Succinate in MDA-MB 231 Triple-Negative Breast Cancer Cells PMCID:    Pubmed No.:31171899 Inhibition of the antigen-presenting ability of dendritic cells by non-structural protein 2 of influenza A virus PMCID:    Pubmed No.:35259598
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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