Product Name :
CHI3L1 polyclonal antibody Background :
Human cartilage glycoprotein 39 (GP-39), also known as YKL-40, is a glycoprotein secreted by articular chondrocytes, synoviocytes and macrophages. Serum and synovial fluid GP-39 levels are elevated in inflammatory diseases and correlate with the degree of joint destruction in rheumatoid arthritis. GP-39 is expressed in articular chondrocytes and synovial cells, as well as in liver, but is undetectable in muscle tissues, lung, pancreas, mononuclear cells and fibroblasts. GP-39 is a candidate autoantigen in rheumatoid arthritis and is important in the capacity of cells to respond to and cope with changes in their environment. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
CHI3L1 polyclonal antibody detects endogenous levels of CHI3L1 protein. Immunogen :
A synthetic peptide corresponding to residues in Human CHI3L1 Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of CHI3L1 pAb at 1:500 dilution Lane1:U-87MG whole cell lysate(40ug) Lane2:L02 whole cell lysate(40ug) Lane3:AML-12 whole cell lysate(40ug) Lane4:C6 whole cell lysate(40ug)

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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