Product Name :
FARSLB polyclonal antibody Background :
FARSLB (Phenylalanine--tRNA ligase beta chain), also known as FARSB, FRSB, PheHB or PheRS, is a 589 amino acid cytoplasmic protein that is the regulatory beta subunit of phenylalanine-tRNA synthetase. A highly conserved member of the aminoacyl-tRNA synthetase class IIc subfamily, FARSLB functions to enzymatically attach L-phenylalanine residues to terminal adenosines on tRNA(Phe). This is an ATP-dependent reaction that yields AMP, a diphosphate and an L-phenylalanine-tagged tRNA(Phe). FARSLB is expressed in brain, heart, kidney, pancreas, placenta and skeletal muscle and its expression is increased in malignant cell lines, suggesting a possible role for FARSLB in carcinogenesis. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
FARSLB polyclonal antibody detects endogenous levels of FARSLB protein. Immunogen :
A synthetic peptide corresponding to residues in Human FARSLB. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of FARSB pAb at 1:500 dilution Lane1:CT-26 whole cell lysate(40ug) Lane2:C6 whole cell lysate(40ug) Lane3:PC3 whole cell lysate(40ug) Lane4:EC9706 whole cell lysate(40ug) Lane5:The pancreas tissue lysate of Mouse(40ug) Lane6:U-87MG whole cell lysate(40ug)

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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