Product Name :
IL-4R/CD124(Phospho-Y497) polyclonal antibody Background :
The armadillo (ARM) repeat family of proteins are related to the Drosophilamelanogaster armadillo protein, a protein essential for wingless signal transduction. ARM proteins are involved in a variety of processes such as cellmigration, cell proliferation, tissue maintenance and tumorigenesis. They areintracellular proteins and function in signal transduction and cell structure. ARMCX3 (armadillo repeat containing, X-linked 3), also known as ALEX3(ARM protein lost in epithelial cancers on chromosome X 3), is a single passmembrane protein belonging to the armadillo repeat family of proteins.ARMCX3 contains three ARM repeats and shares 60% sequence similaritywith the related proteins, ARMCX1 and ARMCX2. ARMCX3 is believed toplay a role in embryonic development and tissue maintenance and may alsofunction as a tumor suppressor. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
IL-4R/CD124(Phospho-Y497) polyclonal antibody detects endogenous levels of IL-4R/CD124 protein only when phosphorylated at Tyr497. Immunogen :
Synthetic phosphopeptide derived from human IL-4R/CD124 around the phosphorylation site of Tyrosine 497. Conjugate :
Unconjugated Modification :
Phosphorylation

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  Western blot (WB) analysis of IL-4R/CD124(Phospho-Y497) polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate(40ug) Lane2:HCT116 whole cell lysate(40ug) Lane3:SGC7901 whole cell lysate(40ug)

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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