Product Name :
IDH3B polyclonal antibody Background :
IDH3B (Isocitrate dehydrogenase 3 (NAD+) b, NAD(+)-specific ICDH) is a 384 amino acid protein encoded by the human gene IDH3B. IDH3B belongs to the isocitrate and isopropylmalate dehydrogenases family and can bind one magnesium or manganese ion per subunit. It is usually found in the mitochondrion as a heterooligomer of subunits a, b, and g in the apparent ratio of 2:1:1. Human NAD-dependent isocitrate dehydrogenase (IDH) is allosterically activated by ADP by lowering the Km for isocitrate. NAD-dependent isocitrate dehydrogenase is a tricarboxylic acid cycle enzyme that produces 2-oxoglutarate, an organic acid required by the glutamine synthetase/glutamate synthase cycle to assimilate ammonium. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
IDH3B polyclonal antibody detects endogenous levels of IDH3B protein. Immunogen :
A synthetic peptide corresponding to residues in Human IDH3B Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of IDH3B pAb at 1:5000 dilution Lane1:H9C2 whole cell lysate(40ug) Lane2:RAW264.7 whole cell lysate(40ug) Lane3:HEK293T whole cell lysate(40ug) Lane4:U-87MG whole cell lysate(40ug)

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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