Product Name :
[KO Validated] RAG2 polyclonal antibody Background :
This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
RAG2 polyclonal antibody detects endogenous levels of RAG2 protein. Immunogen :
Recombinant fusion protein containing a sequence corresponding to amino acids 258-527 of human RAG2 Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot analysis of extracts of various cell lines, using RAG2 antibody at 1:1000 dilution.
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  Western blot analysis of extracts from normal (control) and RAG2 Rabbit pAb knockout (KO) 293T cells, using RAG2 Rabbit pAb antibody at 1:500 dilution.
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  Western blot analysis of extracts from normal (control) and RAG2 Rabbit pAb knockout (KO) 293T cells, using RAG2 Rabbit pAb antibody at 1:500 dilution.
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  Western blot analysis of extracts from normal (control) and RAG2 Rabbit pAb knockout (KO) 293T cells, using RAG2 Rabbit pAb antibody at 1:500 dilution.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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