Product Name :
MAPKAPK-2 (phospho Ser272) Polyclonal Antibody Background :
mitogen-activated protein kinase-activated protein kinase 2(MAPKAPK2) Homo sapiens This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008], Product :
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Phospho-MAPKAPK-2 (S272) Polyclonal Antibody detects endogenous levels of MAPKAPK-2 protein only when phosphorylated at S272. Immunogen :
The antiserum was produced against synthesized peptide derived from human MAPKAPK2 around the phosphorylation site of Ser272. AA range:238-287 Conjugate :
Unconjugated Modification :
Phosphorylation

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  Western blot analysis of lysates from RAW264.7 cells treated with UV 15', using MAPKAPK2 (Phospho-Ser272) Antibody. The lane on the right is blocked with the phospho peptide.
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  Immunohistochemistry analysis of paraffin-embedded human brain, using MAPKAPK2 (Phospho-Ser272) Antibody. The picture on the right is blocked with the phospho peptide.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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