Product Name :
Epsin 3 Polyclonal Antibody Background :
induction:In keratinocytes, by wounding or contact with collagen.,similarity:Belongs to the epsin family.,similarity:Contains 1 ENTH (epsin N-terminal homology) domain.,similarity:Contains 2 UIM (ubiquitin-interacting motif) repeats.,subcellular location:Concentrated in the perinuclear region and associated with clathrin-coated vesicles close to the cell periphery. May shuttle to the nucleus.,tissue specificity:Detected in migrating keratinocytes from wounded skin, but not in differentiating keratinocytes or in normal skin. Detected in chronic wounds, basal cell carcinoma and ulcerative colitis., Product :
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Epsin 3 Polyclonal Antibody detects endogenous levels of Epsin 3 protein. Immunogen :
The antiserum was produced against synthesized peptide derived from human EPN3. AA range:401-450 Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot analysis of lysates from K562 cells, using EPN3 Antibody. The lane on the right is blocked with the synthesized peptide.
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  Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using EPN3 Antibody. The picture on the right is blocked with the synthesized peptide.
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  Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using EPN3 Antibody. The picture on the right is blocked with the synthesized peptide.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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