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RGS16(Ab-168)polyclonal antibody BS65219
  • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using RGS16 (Phospho-Tyr168) Antibody
  • Western blot analysis of lysates from COS7 cells treated with heat shock, using RGS16 (Phospho-Tyr168) Antibody. The lane on the right is blocked with the phospho peptide.
Product NameRGS16(Ab-168)polyclonal antibody
Catalog No.BS65219
Swiss-ProtO15492
Host Rabbit
ReactivityHuman
ApplicationsWB,ELISA
Application_allWestern Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
BiowMW~ 30 kDa
Alternative NameHMGN1; HMG14; Non-histone chromosomal protein HMG-14; High mobility group nucleosome-binding domain-containing protein 1
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50ul $208
100ul $358
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Product Name :
RGS16(Ab-168)polyclonal antibody
Background :
regulator of G-protein signaling 16(RGS16) Homo sapiens The protein encoded by this gene belongs to the 'regulator of G protein signaling' family. It inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits. It also may play a role in regulating the kinetics of signaling in the phototransduction cascade. [provided by RefSeq, Jul 2008],
Product :
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
Phospho-RGS16 (Y168) Polyclonal Antibody detects endogenous levels of RGS16 protein only when phosphorylated at Y168.
Immunogen :
The antiserum was produced against synthesized peptide derived from human HMG14 around the phosphorylation site of Ser21. AA range:10-59
Conjugate :
Unconjugated
Modification :
Unmodification
  • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using RGS16 (Phospho-Tyr168) Antibody
  • Western blot analysis of lysates from COS7 cells treated with heat shock, using RGS16 (Phospho-Tyr168) Antibody. The lane on the right is blocked with the phospho peptide.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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