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p47 phox(Ab-328)polyclonal antibody BS65215
  • Western Blot analysis of various cells using p47-phox Polyclonal Antibody
  • Western Blot analysis of COLO205 cells using p47-phox Polyclonal Antibody
  • Western Blot analysis of COLO205 cells using p47-phox Polyclonal Antibody
  • Western Blot analysis of COLO205 cells using p47-phox Polyclonal Antibody
Product Namep47 phox(Ab-328)polyclonal antibody
Catalog No.BS65215
Swiss-ProtP14598
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB,IHC-p,IF(paraffin section),ELISA
Application_allWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
BiowMW~ 44 kDa
Alternative NameNCF1; NOXO2; SH3PXD1A; Neutrophil cytosol factor 1; NCF-1; 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NCF-47K; Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox-organizing protein 2; SH3
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50ul $208
100ul $358
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Product Name :
p47 phox(Ab-328)polyclonal antibody
Background :
neutrophil cytosolic factor 1(NCF1) Homo sapiens The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq, Jul 2008],
Product :
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
p47-phox Polyclonal Antibody detects endogenous levels of p47-phox protein.
Immunogen :
The antiserum was produced against synthesized peptide derived from human p47 phox. AA range:341-390
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western Blot analysis of various cells using p47-phox Polyclonal Antibody
  • Western Blot analysis of COLO205 cells using p47-phox Polyclonal Antibody
  • Western Blot analysis of COLO205 cells using p47-phox Polyclonal Antibody
  • Western Blot analysis of COLO205 cells using p47-phox Polyclonal Antibody
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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