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GUF1 polyclonal antibody BS65163
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using GUF1 Antibody. The picture on the right is blocked with the synthesized peptide.
Product NameGUF1 polyclonal antibody
Catalog No.BS65163
Swiss-ProtQ8N442
Host Rabbit
ReactivityHuman
ApplicationsIHC-p,IF(paraffin section),ELISA
Application_allImmunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
BiowMW/
Alternative NameGUF1; Translation factor GUF1; mitochondrial; Elongation factor 4 homolog; EF-4; GTPase GUF1; Ribosomal back-translocase
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50ul $158
100ul $275
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Product Name :
GUF1 polyclonal antibody
Background :
GUF1 homolog, GTPase(GUF1) Homo sapiens This gene encodes a GTPase that triggers back-translocation of the elongating ribosome during mitochondrial protein synthesis. The protein contains a highly conserved C-terminal domain not found in other GTPases that facilitates tRNA binding. The encoded protein is thought to prevent misincorporation of amino acids in stressful, suboptimal conditions. An allelic variant in this gene has been associated with early infantile epileptic encephalopathy-40. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016],
Product :
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
GUF1 Polyclonal Antibody detects endogenous levels of GUF1 protein.
Immunogen :
The antiserum was produced against synthesized peptide derived from human GUF1. AA range:421-470
Conjugate :
Unconjugated
Modification :
Unmodification
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using GUF1 Antibody. The picture on the right is blocked with the synthesized peptide.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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