Product Name :
PER2/Period circadian protein 2 Polyclonal Antibody Background :
PER2, a mammalian homologue of the Drosophila period gene, shares a 40% homology with PER1 including the protein dimerization PAS domain. PER2 is a circadian regulator that may act as a transcription factor. It behaves as a negative element in circadian transcriptional loop. PER2 does not appear to bind DNA, suggesting indirect transcriptional inhibition. Expression oscillates with a 24 hour rhythm in the suprachiasmatic nucleus (SCN) and the whole eyes. Oscillations are maintained under constant darkness and are responsive to changes of the light/dark cycles. There is a 4 hour time delay between PER1 and PER2 oscillations. The expression rhythms appear to originate from retina. Product :
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage&Stability :
Store at 4°C short term. Aliquot and store at -51°C long term. Avoid freeze-thaw cycles. Specificity :
PER2/Period circadian protein 2 Polyclonal Antibody detects endogenous levels of PER2/Period circadian protein 2 protein. Immunogen :
KLH conjugated synthetic peptide derived from human PER2:751-850/1225 Conjugate :
Unconjugated Modification :
Unmodification

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  Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PER2) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C
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  Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PER2) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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