Product Name :
HSP70/HSPA1 polyclonal antibody Background :
This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
HSP70/HSPA1 polyclonal antibody detects endogenous levels of HSP70/HSPA1 protein. Immunogen :
A synthetic peptide corresponding to a sequence within amino acids 500-600 of human HSP70/HSPA1 (NP_005336.3). Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot analysis of extracts of various cell lines, using HSP70/HSPA1 antibody at 1:3000 dilution.
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  Immunohistochemistry of paraffin-embedded human lung cancer using HSP70/HSPA1 antibody at dilution of 1:100 (40x lens).
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  Immunohistochemistry of paraffin-embedded human lung cancer using HSP70/HSPA1 antibody at dilution of 1:100 (40x lens).
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  Immunohistochemistry of paraffin-embedded human lung cancer using HSP70/HSPA1 antibody at dilution of 1:100 (40x lens).

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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