Product Name :
IL6R polyclonal antibody Background :
IL-6 activates intracellular signaling through binding a receptor consisting of a ligand-binding protein (IL-6Rα) and a second protein. IL-6 first binds to IL-6Rα (also known as gp80), which subsequently associates with a gp130 dimer. The active signaling complex consists of, at minimum, IL-6, IL-6Rα and a dimer of two gp130 proteins that are linked by a disulfide bond. A soluble form of IL-6Rα, namely sIL-6Rα, is generated by proteolytic cleavage of the membrane-bound precursor and can function as an agonistic molecule that can actively participate in cell-to-cell signaling. The second subunit of the IL-6 complex, gp130, also functions as a component of several additional receptor complexes, including leukemia inhibitory factor (LIF), oncostatin M (OSM), ciliary neurotrophic factor (CNTF) and IL-11. LIF binds to the LIF receptor with low affinity and to a complex of the LIF receptor and gp130 with high affinity, while OSM appears to bind to gp130 with low affinity and to a complex of gp130 and the LIF receptor with high affinity. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
IL6R polyclonal antibody detects endogenous levels of IL6R protein. Immunogen :
Synthetic peptide, corresponding to Human IL6R. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of IL6R polyclonal antibody at 1:500 dilution LaneA:HEK293T whole cell lysate(40ug) LaneB:Hela whole cell lysate(40ug) LaneC:HGC27 whole cell lysate(40ug) LaneD:The Liver tissue lysate of Mouse(40ug) LaneE:The Lung tissue lysate of Mouse(40ug) LaneF:The Liver tissue lysate of Rat(40ug) LaneG:The Lung tissue lysate of Rat(40ug)

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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