Product Name :
PER2 polyclonal antibody Background :
Biological timepieces called circadian Clocks are responsible for the regulationof hormonal rhythms, sleep cycles and other behaviors. The superchiasmaticnucleus (SCN), which is located in the brain, was the first mammalian circadian Clock to be discovered. A number of transcription factors appearing to bemolecular components of the SCN Clock have been identified. Mutations within the Clock gene increase the length of the endogenous period and cause aloss of rhythmicity of circadian oscillations. Three mammalian period proteins, designated Per1, Per2 and Per3, exhibit circadian rhythyms in the SCN. Duringsubjective night, Per1 and Per2 RNA levels increase in response to light pulses while Per3 RNA levels show no change in response to light pulses. Tim, fortimeless, interacts with Per1 as well as Per2; and Tim and Per1 negativelyregulate Clock-BMAL1-induced transcription. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
PER2 polyclonal antibody detects endogenous levels of PER2 protein. Immunogen :
Synthetic peptide, corresponding to Human PER2. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of PER2 polyclonal antibody at 1:1000 dilution Lane1:A549 whole cell lysate(40ug) Lane2:U-87MG whole cell lysate(40ug)

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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