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ERK1/2 (Y204) polyclonal antibody AP0491
  • Western blot (WB) analysis of ERK1/2 (Y204) pAb at 1:1000 dilution Lane1:C6 whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:The Brain tissue lysate of Rat(40ug) Lane4:K562 whole cell lysate(40ug)
  • Immunofluorescence analysis of C6 cells using ERK1/2 (Y204) antibody at dilution of 1:50.
Product NameERK1/2 (Y204) polyclonal antibody
Catalog No.AP0491
Swiss-ProtP27361/P28482
Host Rabbit
ReactivityHuman,Rat
ApplicationsWB IF
Application_allWB: 1:1000~1:2000 IF: 1:50~1:200
BiowMW~ 42, 44 kDa
Alternative NameMitogen-activated protein kinase 3; MAP kinase 3; MAPK 3; ERT2; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; p44-MAPK; Microtubule-associated protein 2 kinase; p44-ERK1; MAPK3; ERK1; PRKM3; Mitogen-activated protein kinase 1; MAP kinase 1; MAPK 1; ERT1; Extracellular signal-regulated kinase 2; ERK-2; MAP kinase isoform p42; p42-MAPK; Mitogen-activated protein kinase 2; MAP kinase 2; MAPK 2; MAPK1; ERK2; PRKM1; PRKM2
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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50ul $158
100ul $275
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Product Name :
ERK1/2 (Y204) polyclonal antibody
Background :
The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at Tyrosine 204 and 187 and Threonine 177 and 160 residues mapping within a characteristic Thr-Glu-Tyr motif. Phosphorylation at both the Threonine 202 and Tyrosine 204 residues of ERK1 and Threonine 185 and Tyrosine 187 residues of ERK2 is required for full enzymatic activation. The structural consequences of dual-phosphorylation in the ERK2 include active site closure, alignment of key catalytic residues that interact with ATP, and remodeling of the activation loop. In response to activation, MAP kinases phosphorylate downstream components on serine and threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
ERK1/2 (Y204) polyclonal antibody detects endogenous levels of ERK1/2 protein.
Immunogen :
Synthetic peptide, corresponding to Human ERK1/2.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot (WB) analysis of ERK1/2 (Y204) pAb at 1:1000 dilution Lane1:C6 whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:The Brain tissue lysate of Rat(40ug) Lane4:K562 whole cell lysate(40ug)
  • Immunofluorescence analysis of C6 cells using ERK1/2 (Y204) antibody at dilution of 1:50.
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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