Contact us : info@bioworlde.com
Home > Product > Primary Antibodies
Stat3 polyclonal antibody AP0365
  • Western blot (WB) analysis of Stat3 pAb at 1:1000 dilution Lane1:H9C2 whole cell lysate(40ug) Lane2:AML-12 whole cell lysate(40ug) Lane3:MCF-7 whole cell lysate(40ug) Lane4:THP-1 whole cell lysate(40ug) Lane5:HepG2 whole cell lysate(40ug)
  • Immunoprecipitation of MCF-7 cell lysates using Stat3 pAb (Sepharose Bead Conjugate)#BD0048 (lane 4 and lane 5) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3) .Lane 1 is 30% input. The western blot was probed using Stat3 pAb #AP0365.
  • Immunoprecipitation of MCF-7 cell lysates using Stat3 pAb (Sepharose Bead Conjugate)#BD0048 (lane 4 and lane 5) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3) .Lane 1 is 30% input. The western blot was probed using Stat3 pAb #AP0365.
Product NameStat3 polyclonal antibody
Catalog No.AP0365
Swiss-ProtP40763
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB IP IF
Application_allWB:1:500~1:1000 IP: 1:50~1:200 IF: 1:50~1:200
BiowMW~ 80,89KDa
Alternative NameSignal transducer and activator of transcription 3; Acute-phase response factor; STAT-3; APRF
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
Browse similar products>>
Size Price
50ul $158
100ul $275
Add to cart My orders
Product Name :
Stat3 polyclonal antibody
Background :
Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of Jak kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-α and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3β appears to be activated by both while Stat3α is activated by EGF, but not by IL-6. Highest expresion of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 has been shown to be activated by prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
STAT3 polyclonal antibody detects endogenous levels of STAT3 protein.
Immunogen :
Recombinant protein.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot (WB) analysis of Stat3 pAb at 1:1000 dilution Lane1:H9C2 whole cell lysate(40ug) Lane2:AML-12 whole cell lysate(40ug) Lane3:MCF-7 whole cell lysate(40ug) Lane4:THP-1 whole cell lysate(40ug) Lane5:HepG2 whole cell lysate(40ug)
  • Immunoprecipitation of MCF-7 cell lysates using Stat3 pAb (Sepharose Bead Conjugate)#BD0048 (lane 4 and lane 5) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3) .Lane 1 is 30% input. The western blot was probed using Stat3 pAb #AP0365.
  • Immunoprecipitation of MCF-7 cell lysates using Stat3 pAb (Sepharose Bead Conjugate)#BD0048 (lane 4 and lane 5) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3) .Lane 1 is 30% input. The western blot was probed using Stat3 pAb #AP0365.
Interleukin-15 receptor-directed immunotoxins atteunuate disease severity in rat adjuvant arthritis PMCID:    Pubmed No.:20188418 Expression of il-23/th17 pathway in a murine model of coxsackie virus b3-induced viral myocarditis PMCID:    Pubmed No.:21672246 Topical application of Pseudolaric acid B improve DNFB-induced contact hypersensitivity via regulating the balance of Th1/Th17/Treg cell subsets PMCID:    Pubmed No.:22266211 Oroxylin A inhibits colitis-associated carcinogenesis through modulating the IL-6/STAT3 signaling pathway. PMCID:    Pubmed No.:23823704 Sesquiterpene dimer (DSF-52) from Artemisia argyi inhibits microglia-mediated neuroinflammation via suppression of NF-κB, JNK/p38 MAPKs and Jak2/Stat3 signaling pathways PMCID:    Pubmed No.:24055519 Hydroxy-safflor yellow A inhibits neuroinflammation mediated by Aβ1–42 in BV-2 cells PMCID:    Pubmed No.:24412680 Bone marrow microenvironment confers imatinib resistance to chronic myelogenous leukemia and oroxylin A reverses the resistance by suppressing Stat3 pathway PMCID:    Pubmed No.:24671465 Activation of Mesenchymal Stem Cells by Macrophages Prompts Human Gastric Cancer Growth through NF-κB Pathway PMCID:    Pubmed No.:24824968 Wogonin suppresses human alveolar adenocarcinoma cell A549 migration in inflammatory microenvironment by modulating the IL‐6/STAT3 signaling pathway PMCID:    Pubmed No.:24976450 The tumor suppressor miR-124 inhibits cell proliferation by targeting STAT3 and functions as a prognostic marker for postoperative NSCLC patients PMCID:    Pubmed No.:25531908 Protective Effects of Calycosin Against CCl4-Induced Liver Injury with Activation of FXR and STAT3 in Mice PMCID:    Pubmed No.:25143196 Scrapie Infection in Experimental Rodents and SMB-S15 Cells Decreased the Brain Endogenous Levels and Activities of Sirt1. PMCID:    Pubmed No.:25391763 Sulindac has strong antifibrotic effects by suppressing STAT3-related miR-21. PMCID:    Pubmed No.:25704671 M2 polarized macrophages induced by CSE promote proliferation, migration, and invasion of alveolar basal epithelial cells PMCID:    Pubmed No.:26253658 Inhibitory Effects of Isorhamnetin on the Invasion of Human Breast Carcinoma Cells by Downregulating the Expression and Activity of Matrix Metalloproteinase-2/9 PMCID:    Pubmed No.:26359917 Interferon-γ and tumor necrosis factor-α promote the ability of human placenta-derived mesenchymal stromal cells to express programmed death ligand-2 and induce the differentiation of CD4(+)interleukin-10(+) and CD8(+)interleukin-10(+)Treg subsets. PMCID:    Pubmed No.:26432559 Targeted anti-colon cancer activities of a millet bran-derived peroxidase were mediated by elevated ROS generation PMCID:    Pubmed No.:26075747 microRNA-23a,-27a and-24 synergistically regulate JAK1/Stat3 cascade and serve as novel therapeutic targets in human acute erythroid leukemia PMCID:    Pubmed No.:27086927 Protective Effects of Calycosin Against CCl4-Induced Liver Injury with Activation of FXR and STAT3 in Mice PMCID:    Pubmed No.:25143196 Scrapie Infection in Experimental Rodents and SMB-S15 Cells Decreased the Brain Endogenous Levels and Activities of Sirt1. PMCID:    Pubmed No.:25391763 Sesquiterpene dimer (DSF-52) from Artemisia argyi inhibits microglia-mediated neuroinflammation via suppression of NF-κB, JNK/p38 MAPKs and Jak2/Stat3 signaling pathways PMCID:    Pubmed No.:24055519 Inhibitory Effects of Isorhamnetin on the Invasion of Human Breast Carcinoma Cells by Downregulating the Expression and Activity of Matrix Metalloproteinase-2/9 PMCID:    Pubmed No.:26359917 Interferon-γ and tumor necrosis factor-α promote the ability of human placenta-derived mesenchymal stromal cells to express programmed death ligand-2 and induce the differentiation of CD4(+)interleukin-10(+) and CD8(+)interleukin-10(+)Treg subsets. PMCID:    Pubmed No.:26432559 Targeted anti-colon cancer activities of a millet bran-derived peroxidase were mediated by elevated ROS generation PMCID:    Pubmed No.:26075747 microRNA-23a,-27a and-24 synergistically regulate JAK1/Stat3 cascade and serve as novel therapeutic targets in human acute erythroid leukemia PMCID:    Pubmed No.:27086927 Activation of Mesenchymal Stem Cells by Macrophages Prompts Human Gastric Cancer Growth through NF-κB Pathway PMCID:    Pubmed No.:24824968 Hydroxy-safflor yellow A inhibits neuroinflammation mediated by Aβ1–42 in BV-2 cells PMCID:    Pubmed No.:24412680 Bone marrow microenvironment confers imatinib resistance to chronic myelogenous leukemia and oroxylin A reverses the resistance by suppressing Stat3 pathway PMCID:    Pubmed No.:24671465 Interferon-γ and tumor necrosis factor-α promote the ability of human placenta–derived mesenchymal stromal cells to express programmed death ligand-2 and induce the differentiation of CD4+interleukin-10+ and CD8+interleukin-10+Treg subsets PMCID:    Pubmed No.:26432559 M2 polarized macrophages induced by CSE promote proliferation, migration, and invasion of alveolar basal epithelial cells PMCID:    Pubmed No.:26253658 Arctigenin exerts anti-colitis efficacy through inhibiting the differentiation of Th1 and Th17 cells via an mTORC1-dependent pathway PMCID:    Pubmed No.:26074264 Sulindac has strong antifibrotic effects by suppressing STAT3‐related miR‐21 PMCID:    Pubmed No.:25704671 FXR antagonism of NSAIDs contributes to drug-induced liver injury identified by systems pharmacology approach PMCID:    Pubmed No.:25631039 Protective effects of alisol B 23-acetate from edible botanical Rhizoma alismatis against carbon tetrachloride-induced hepatotoxicity in mice PMCID:    Pubmed No.:25747392 The tumor suppressor miR-124 inhibits cell proliferation by targeting STAT3 and functions as a prognostic marker for postoperative NSCLC patients PMCID:    Pubmed No.:25531908 Oral Administration of Lactobacillus rhamnosus GG Ameliorates Salmonella Infantis-Induced Inflammation in a Pig Model via Activation of the IL-22BP/IL-22/STAT3 Pathway PMCID:    Pubmed No.:28770173 Scrapie Infection in Experimental Rodents and SMB-S15 Cells Decreased the Brain Endogenous Levels and Activities of Sirt1 PMCID:    Pubmed No.:25391763 Inhibitory Effects of Isorhamnetin on the Invasion of Human Breast Carcinoma Cells by Downregulating the Expression and Activity of Matrix Metalloproteinase-2/9 PMCID:    Pubmed No.:26359917 Epigallocatechin‑3‑gallate‑induced inhibition of interleukin‑6 release and adjustment of the regulatory T/T helper 17 cell balance in the treatment of colitis in mice PMCID:    Pubmed No.:26668622 Interleukin-6 suppression reduces tumour self-seeding by circulating tumour cells in a human osteosarcoma nude mouse model PMCID:    Pubmed No.:26623559 IL-9-triggered lncRNA Gm13568 regulates Notch1 in astrocytes through interaction with CBP/P300: contribute to the pathogenesis of experimental autoimmune encephalomyelitis PMCID:    Pubmed No.:33971906 MiR‐409‐3p and MiR‐1896 co‐operatively participate in IL‐17‐induced inflammatory cytokine production in astrocytes and pathogenesis of EAE mice via targeting SOCS3/STAT3 signaling PMCID:    Pubmed No.:30294880 Angelicin Alleviates Post-Trauma Osteoarthritis Progression by Regulating Macrophage Polarization via STAT3 Signaling Pathway PMCID:    Pubmed No.:34177582 Th9 cytokines curb cervical cancer progression and immune evasion PMCID:    Pubmed No.:31563404 Andrographolide Inhibition of Th17-Regulated Cytokines and JAK1/STAT3 Signaling in OVA-Stimulated Asthma in Mice PMCID:    Pubmed No.:34194525 Combination of RSK inhibitor LJH-685 and FLT3 inhibitor FF-10101 promoted apoptosis and proliferation inhibition of AML cell lines PMCID:    Pubmed No.:36036884
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
COPYRIGHT © 2015-2018 Bioworld Technology, Inc. All rights reserved POLYCLONAL AND MONOCLONAL ANTIBODY CENTER