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ERK1/2 (T202/Y204) polyclonal antibody AP0485
  • Western blot (WB) analysis of ERK1/2 pAb at 1:500 dilution Lane1:U-87MG whole cell lysate(40ug) Lane2:H9C2 whole cell lysate(40ug) Lane3:EC9706 whole cell lysate(40ug)
  • Western blot (WB) analysis of ERK1/2 (T202/Y204) pAb at 1:500 dillution Lane1:Hela whole cell lysate(20μg) Lane2:NIH-3T3 whole cell lysate(40μg) Lane3:H9C2 whole cell lysate(40μg)
  • Western blot (WB) analysis of ERK1/2 (T202/Y204) pAb at 1:500 dillution Lane1:Hela whole cell lysate(20μg) Lane2:NIH-3T3 whole cell lysate(40μg) Lane3:H9C2 whole cell lysate(40μg)
Product NameERK1/2 (T202/Y204) polyclonal antibody
Catalog No.AP0485
Swiss-ProtP27361/P28482
Host Rabbit
ReactivityHuman,Rat,Mouse
ApplicationsWB IF
Application_allWB: 1:500~1:1000 IF: 1:50~1:200
BiowMW~ 45 kDa
Alternative NameMitogen-activated protein kinase 3; MAP kinase 3; MAPK 3; ERT2; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; p44-MAPK; Microtubule-associated protein 2 kinase; p44-ERK1; MAPK3; ERK1; PRKM3; Mitogen-activated protein kinase 1; MAP kinase 1; MAPK 1; ERT1; Extracellular signal-regulated kinase 2; ERK-2; MAP kinase isoform p42; p42-MAPK; Mitogen-activated protein kinase 2; MAP kinase 2; MAPK 2; MAPK1; ERK2; PRKM1; PRKM2
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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100ul $275
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Product Name :
ERK1/2 (T202/Y204) polyclonal antibody
Background :
The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at specific tyrosine and Threonine sites mapping within a characteristic Thr- Glu-Tyr motif. Phosphorylation at both the Thr and Tyr residues is required for full enzymatic activation. In response to activation, MAP kinases phos-phorylate downstream components on Serine and Threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
ERK1/2 (T202/Y204) polyclonal antibody detects endogenous levels of ERK1/2 protein.
Immunogen :
Synthetic peptide, corresponding to amino acids 180-220 of Human ERK1.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot (WB) analysis of ERK1/2 pAb at 1:500 dilution Lane1:U-87MG whole cell lysate(40ug) Lane2:H9C2 whole cell lysate(40ug) Lane3:EC9706 whole cell lysate(40ug)
  • Western blot (WB) analysis of ERK1/2 (T202/Y204) pAb at 1:500 dillution Lane1:Hela whole cell lysate(20μg) Lane2:NIH-3T3 whole cell lysate(40μg) Lane3:H9C2 whole cell lysate(40μg)
  • Western blot (WB) analysis of ERK1/2 (T202/Y204) pAb at 1:500 dillution Lane1:Hela whole cell lysate(20μg) Lane2:NIH-3T3 whole cell lysate(40μg) Lane3:H9C2 whole cell lysate(40μg)
Dose-dependent effect of tamoxifen in tamoxifen-resistant breast cancer cells via stimulation by the ERK1/2 and AKT signaling pathways. PMCID:    Pubmed No.:23338782 Dihydrotestosterone alters urocortin levels in human umbilical vein endothelial cells. PMCID:    Pubmed No.:23801677 Lipoxin A4-induced heme oxygenase-1 protects cardiomyocytes against hypoxia/reoxygenation injury via p38 MAPK activation and Nrf2/ARE complex. PMCID:    Pubmed No.:23826208 An apple oligogalactan suppresses endotoxin-induced cyclooxygenase-2 expression by inhibition of LPS pathways PMCID:    Pubmed No.:23827762 MicroRNA-200a regulates Grb2 and suppresses differentiation of mouse embryonic stem cells into endoderm and mesoderm. PMCID:    Pubmed No.:23874841 The stimulation of osteogenic differentiation of mesenchymal stem cells and vascular endothelial growth factor secretion of endothelial cells by β-CaSiO3/β-Ca3(PO4)2 scaffolds PMCID:    Pubmed No.:23894078 Grape Seed Procyanidin Reversal of P-glycoprotein Associated Multi-Drug Resistance via Down-regulation of NF-κB and MAPK/ERK Mediated YB-1 Activity in A2780/T Cells PMCID:    Pubmed No.:23967153 Self-assembling nanofibers improve cognitive impairment in a transgenic mice model of Alzheimer's disease PMCID:    Pubmed No.:24103374 Musashi2 modulates K562 leukemic cell proliferation and apoptosis involving the MAPK pathway PMCID:    Pubmed No.:24076374 ZFX knockdown inhibits growth and migration of non-small cell lung carcinoma cell line H1299 PMCID:    Pubmed No.:24228108 Knockdown of protein tyrosine phosphatase receptor U inhibits growth and motility of gastric cancer cells PMCID:    Pubmed No.:25337216 Modulatory Effects of Vasoactive Intestinal Peptide on Intestinal Mucosal Immunity and Microbial Community of Weaned Piglets Challenged by an Enterotoxigenic Escherichia coli (K88). PMCID:    Pubmed No.:25101851 Expression of ERK and p-ERK proteins of ERK signaling pathway in the kidneys of fluoride-exposed carp (Cyprinus carpio). PMCID:    Pubmed No.:25190107 Ubiquitin‑specific protease 22‑induced autophagy is correlated with poor prognosis of pancreatic cancer PMCID:    Pubmed No.:25241857 Diosgenin inhibits superoxide generation in FMLP-activated mouse neutrophils via multiple pathways PMCID:    Pubmed No.:25246240 MSTN, mTOR and FoxO4 Are Involved in the Enhancement of Breast Muscle Growth by Methionine in Broilers with Lower Hatching Weight PMCID:    Pubmed No.:25437444 Role of the MAPK pathway in the observed bystander effect in lymphocytes co-cultured with macrophages irradiated with γ-rays or carbon ions PMCID:    Pubmed No.:25748424 Epigallocatechin-3-gallate attenuates lipopolysaccharide-induced mastitis in rats via suppressing MAPK mediated inflammatory responses and oxidative stress PMCID:    Pubmed No.:25840281 Inhibitory effects of Chikusetsusaponin IVa on lipopolysaccharide-induced pro-inflammatory responses in THP-1 cells. PMCID:    Pubmed No.:26157065 Fluid Shear Stress Inhibits TNF-α-induced Osteoblast Apoptosis via ERK5 Signaling Pathway PMCID:    Pubmed No.:26325467 8-Methoxypsoralen Induces Intrinsic Apoptosis in HepG2 Cells: Involvement of Reactive Oxygen Species Generation and ERK1/2 Pathway Inhibition PMCID:    Pubmed No.:26316083 Phloretin induces apoptosis of non-small cell lung carcinoma A549 cells via JNK1/2 and p38 MAPK pathways PMCID:    Pubmed No.:26503828 DGAEE, a newly synthesized derivative of glycyrrhetinic acid, potently attenuates mouse septic shock via its main metabolite DGA in an IL-10-dependent manner PMCID:    Pubmed No.:26456500 A novel acetylcholinesterase inhibitor and calcium channel blocker SCR-1693 improves Aβ25–35-impaired mouse cognitive function PMCID:    Pubmed No.:26554390 Lipoxin A4 Attenuates Cell Invasion by Inhibiting ROS/ERK/MMP Pathway in Pancreatic Cancer PMCID:    Pubmed No.:26649143 Overexpression of miR-335 confers cell proliferation and tumour growth to colorectal carcinoma cells PMCID:    Pubmed No.:26708214 Dioscin suppresses human laryngeal cancer cells growth via induction of cell-cycle arrest and MAPK-mediated mitochondrial-derived apoptosis and inhibition of tumor invasion. PMCID:    Pubmed No.:26849940 MicroRNA-9 regulates cardiac fibrosis by targeting PDGFR-β in rats PMCID:    Pubmed No.:26896308 Dynamic monitoring of GPER-mediated estrogenic effects in breast cancer associated fibroblasts: an alternative role of estrogen in mammary carcinoma development PMCID:    Pubmed No.:27016131 TNF‐α downregulates CIDEC via MEK/ERK pathway in human adipocytes PMCID:    Pubmed No.:27062372 Fluid shear stress suppresses TNF-α-induced apoptosis in MC3T3-E1 cells: Involvement of ERK5-AKT-FoxO3a-Bim/FasL signaling pathways PMCID:    Pubmed No.:27060196 Knockdown of protein tyrosine phosphatase receptor U inhibits growth and motility of gastric cancer cells PMCID:    Pubmed No.:25337216 Modulatory Effects of Vasoactive Intestinal Peptide on Intestinal Mucosal Immunity and Microbial Community of Weaned Piglets Challenged by an Enterotoxigenic Escherichia coli (K88). PMCID:    Pubmed No.:25101851 Expression of ERK and p-ERK proteins of ERK signaling pathway in the kidneys of fluoride-exposed carp (Cyprinus carpio). PMCID:    Pubmed No.:25190107 Ubiquitin‑specific protease 22‑induced autophagy is correlated with poor prognosis of pancreatic cancer PMCID:    Pubmed No.:25241857 Diosgenin inhibits superoxide generation in FMLP-activated mouse neutrophils via multiple pathways PMCID:    Pubmed No.:25246240 MSTN, mTOR and FoxO4 Are Involved in the Enhancement of Breast Muscle Growth by Methionine in Broilers with Lower Hatching Weight PMCID:    Pubmed No.:25437444 Self-assembling nanofibers improve cognitive impairment in a transgenic mice model of Alzheimer's disease PMCID:    Pubmed No.:24103374 Musashi2 modulates K562 leukemic cell proliferation and apoptosis involving the MAPK pathway PMCID:    Pubmed No.:24076374 ZFX knockdown inhibits growth and migration of non-small cell lung carcinoma cell line H1299 PMCID:    Pubmed No.:24228108 Phloretin induces apoptosis of non-small cell lung carcinoma A549 cells via JNK1/2 and p38 MAPK pathways PMCID:    Pubmed No.:26503828 DGAEE, a newly synthesized derivative of glycyrrhetinic acid, potently attenuates mouse septic shock via its main metabolite DGA in an IL-10-dependent manner PMCID:    Pubmed No.:26456500 A novel acetylcholinesterase inhibitor and calcium channel blocker SCR-1693 improves Aβ25–35-impaired mouse cognitive function PMCID:    Pubmed No.:26554390 Lipoxin A4 Attenuates Cell Invasion by Inhibiting ROS/ERK/MMP Pathway in Pancreatic Cancer PMCID:    Pubmed No.:26649143 Overexpression of miR-335 confers cell proliferation and tumour growth to colorectal carcinoma cells PMCID:    Pubmed No.:26708214 Dioscin suppresses human laryngeal cancer cells growth via induction of cell-cycle arrest and MAPK-mediated mitochondrial-derived apoptosis and inhibition of tumor invasion. PMCID:    Pubmed No.:26849940 MicroRNA-9 regulates cardiac fibrosis by targeting PDGFR-β in rats PMCID:    Pubmed No.:26896308 Dynamic monitoring of GPER-mediated estrogenic effects in breast cancer associated fibroblasts: an alternative role of estrogen in mammary carcinoma development PMCID:    Pubmed No.:27016131 TNF‐α downregulates CIDEC via MEK/ERK pathway in human adipocytes PMCID:    Pubmed No.:27062372 Fluid shear stress suppresses TNF-α-induced apoptosis in MC3T3-E1 cells: Involvement of ERK5-AKT-FoxO3a-Bim/FasL signaling pathways PMCID:    Pubmed No.:27060196 Fluid shear stress suppresses TNF-α-induced apoptosis in MC3T3-E1 cells: Involvement of ERK5-AKT-FoxO3a-Bim/FasL signaling pathways PMCID:    Pubmed No.:27060196 Aspirin suppresses TNF‑α‑induced MMP‑9 expression via NF‑κB and MAPK signaling pathways in RAW264.7 cells PMCID:    Pubmed No.:29285098 PKG II reverses HGF-triggered cellular activities by phosphorylating serine 985 of c-Met in gastric cancer cells PMCID:    Pubmed No.:27147579 The antimetastatic effect and underlying mechanisms of thioredoxin reductase inhibitor ethaselen PMCID:    Pubmed No.:30496814 Tropomodulin1 Expression Increases Upon Maturation in Dendritic Cells and Promotes Their Maturation and Immune Functions PMCID:    Pubmed No.:33552047 Hsa-let-7c controls the committed differentiation of IGF-1-treated mesenchymal stem cells derived from dental pulps by targeting IGF-1R via the MAPK pathways PMCID:    Pubmed No.:29650947 The anti-androgenic effects of cypermethrin mediated by non-classical testosterone pathway activation of mitogen-activated protein kinase cascade in mouse Sertoli cells PMCID:    Pubmed No.:30974244 Anti-neuroinflammatory Effect of Short-Chain Fatty Acid Acetate against Alzheimer’s Disease via Upregulating GPR41 and Inhibiting ERK/JNK/NF-κB PMCID:    Pubmed No.:32583667 Identification of extracellular vesicles-transported miRNAs in Erlotinib-resistant head and neck squamous cell carcinoma PMCID:    Pubmed No.:32157550 A novel biscoumarin derivative dephosphorylates ERK and alleviates apoptosis induced by mitochondrial oxidative damage in ischemic stroke mice PMCID:    Pubmed No.:33141045 A Biomimicking Polymeric Cryogel Scaffold for Repair of Critical-Sized Cranial Defect in a Rat Model PMCID:    Pubmed No.:30950322 Estrogen promotes the metastasis of non‑small cell lung cancer via estrogen receptor β by upregulation of Toll‑like receptor 4 and activation of the myd88/NF‑κB/MMP2 pathway PMCID:    Pubmed No.:32364243 Traditional Chinese Medicine CFF‐1 induced cell growth inhibition, autophagy, and apoptosis via inhibiting EGFR‐related pathways in prostate cancer PMCID:    Pubmed No.:29533017 Traditional Chinese Medicine CFF‐1 induced cell growth inhibition, autophagy, and apoptosis via inhibiting EGFR‐related pathways in prostate cancer PMCID:    Pubmed No.:29533017 Protective effect of bone marrow mesenchymal stem cell-derived exosomes against the reproductive toxicity of cyclophosphamide is associated with the p38MAPK/ERK and AKT signaling pathways PMCID:    Pubmed No.:33565424 Sub‐chronic administration of benzo[a]pyrene disrupts hippocampal long‐term potentiation via inhibiting CaMK II/PKC/PKA‐ERK‐CREB signaling in rats PMCID:    Pubmed No.:32255272 Let-7b regulates the adriamycin resistance of chronic myelogenous leukemia by targeting AURKB in K562/ADM cells PMCID:    Pubmed No.:32856506 Activation of RAW264.7 macrophage by Exopolysaccharide from Aphanothece halaphytica (EPSAH) and the underlying mechanisms PMCID:    Pubmed No.:32083746 4‐Sulfonyloxy/alkoxy benzoxazolone derivatives with high anti‐inflammatory activities: Synthesis, biological evaluation, and action mechanism via p38/ERK‐NF‐κB/iNOS pathway PMCID:    Pubmed No.:32915501 -Hederin Arrests Cell Cycle at G2/M Checkpoint and Promotes Mitochondrial Apoptosis by Blocking Nuclear Factor-B Signaling in Colon Cancer Cells PMCID:    Pubmed No.:30363706 Cross regulation of signaling pathways in gastrointestinal stromal tumor PMCID:    Pubmed No.:30405821 L-Theanine Down-Regulates the JAK/STAT3 Pathway to Attenuate the Proliferation and Migration of Vascular Smooth Muscle Cells Induced by Angiotensin II PMCID:    Pubmed No.:30381668 EGFR‑associated pathways involved in traditional Chinese medicine (TCM)‑1‑induced cell growth inhibition, autophagy and apoptosis in prostate cancer PMCID:    Pubmed No.:29620175 Zurampic Protects Pancreatic β-Cells from High Uric Acid Induced-Damage by Inhibiting URAT1 and Inactivating the ROS/AMPK/ERK Pathways PMCID:    Pubmed No.:29843128 Puerarin alleviates depressive-like behaviors in high-fat diet-induced diabetic mice via modulating hippocampal GLP-1R/BDNF/TrkB signaling PMCID:    Pubmed No.:36039913 Delivery of miR-3529-3p using MnO2-SiO2-APTES nanoparticles combined with phototherapy suppresses lung adenocarcinoma progression by targeting HIGD1A PMCID:    Pubmed No.:36808485
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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