Stat1 polyclonal antibody 
Datasheet
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Product Name :
Stat1 polyclonal antibody Background :
Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Stat1 polyclonal antibody detects endogenous levels of Stat1 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 680-720 of Human Stat1. Conjugate :
Unconjugated Modification :
Unmodification
Stat1 polyclonal antibody Background :
Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Stat1 polyclonal antibody detects endogenous levels of Stat1 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 680-720 of Human Stat1. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of Stat1 polyclonal antibody at 1:1000 dillution Lane1:NIH-3T3 ) whole cell lysate(40μg) Lane2:NIH-3T3 treated with UV(4h) whole cell lysate(40μg) Lane3:NIH-3T3 treated with PMA(60ng/ml, 30min) whole cell lysate(40μg) Lane4:Hela whole cell lysate(40μg) Lane5:Hela treated with IFN-γ (100ng/ml,15min) whole cell lysate(40μg) Lane6:Hela treated with PMA(60ng/ml, 30min) whole cell lysate(40μg) -
Immunohistochemistry (IHC) analyzes of Stat1 pAb in paraffin-embedded human colorectal cancer carcinoma tissue at 1:100. -
Immunohistochemistry (IHC) analyzes of Stat1 pAb in paraffin-embedded human colorectal cancer carcinoma tissue at 1:100. -
Immunohistochemistry (IHC) analyzes of Stat1 pAb in paraffin-embedded human colorectal cancer carcinoma tissue at 1:100.
Local hyperthermia could induce antiviral activity by endogenous interferon-dependent pathway in condyloma acuminata
PMCID: Pubmed No.:20797409
STAT1 negatively regulates hepatocellular carcinoma cell proliferation
PMCID: Pubmed No.:23588992
Human Interleukin 23 Receptor Induces Cell Apoptosis in Mammalian Cells by Intrinsic Mitochondrial Pathway Associated with the Down-Regulation of RAS/Mitogen-Activated Protein Kinase and Signal Transducers and Activators of Transcription factor 3 Signaling Pathways
PMCID: Pubmed No.:24351840
The Effects of Fludarabine on Rat Cerebral Ischemia
PMCID: Pubmed No.:24825839
Interferon-γ and tumor necrosis factor-α promote the ability of human placenta-derived mesenchymal stromal cells to express programmed death ligand-2 and induce the differentiation of CD4(+)interleukin-10(+) and CD8(+)interleukin-10(+)Treg subsets.
PMCID: Pubmed No.:26432559
STAT1 inhibits human hepatocellular carcinoma cell growth through induction of p53 and Fbxw7
PMCID: Pubmed No.:26617467
Proteomic analysis of sheep primary testicular cells infected with bluetongue virus
PMCID: Pubmed No.:26989863
Interferon-γ and tumor necrosis factor-α promote the ability of human placenta-derived mesenchymal stromal cells to express programmed death ligand-2 and induce the differentiation of CD4(+)interleukin-10(+) and CD8(+)interleukin-10(+)Treg subsets.
PMCID: Pubmed No.:26432559
STAT1 inhibits human hepatocellular carcinoma cell growth through induction of p53 and Fbxw7
PMCID: Pubmed No.:26617467
Proteomic analysis of sheep primary testicular cells infected with bluetongue virus
PMCID: Pubmed No.:26989863
The Effects of Fludarabine on Rat Cerebral Ischemia
PMCID: Pubmed No.:24825839
Human Interleukin 23 Receptor Induces Cell Apoptosis in Mammalian Cells by Intrinsic Mitochondrial Pathway Associated with the Down-Regulation of RAS/Mitogen-Activated Protein Kinase and Signal Transducers and Activators of Transcription factor 3 Signaling Pathways
PMCID: Pubmed No.:24351840
Interferon-γ and tumor necrosis factor-α promote the ability of human placenta–derived mesenchymal stromal cells to express programmed death ligand-2 and induce the differentiation of CD4+interleukin-10+ and CD8+interleukin-10+Treg subsets
PMCID: Pubmed No.:26432559
iTRAQ protein profile analysis of neuroblastoma (NA) cells infected with the rabies viruses rHep-Flury and Hep-dG
PMCID: Pubmed No.:26217322
STAT1 inhibits human hepatocellular carcinoma cell growth through induction of p53 and Fbxw7
PMCID: Pubmed No.:26617467
Resveratrol Promoted Interferon-α-Induced Growth Inhibition and Apoptosis of SMMC7721 Cells by Activating the SIRT/STAT1
PMCID: Pubmed No.:29762078
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.