Product Name :
MAPKAPK-2 (Phospho-T334) polyclonal antibody Background :
In response to cytokines, stress, and chemotactic factors, MAP kinase-activated protein kinase 2 (MAPKAPK-2) is rapidly phosphorylated and activated. It has been shown that MAPKAPK-2 is a direct target of p38 MAPK. Multiple residues of MAPKAPK-2 are phosphorylated in vivo in response to stress. However, only four residues (Thr25, Thr222, Ser272, and Thr334) are phosphorylated by p38 MAPK in an in vitro kinase assay. Phosphorylation at Thr222, Ser272, and Thr334 appears to be essential for the activity of MAPKAPK-2. Thr25 is phosphorylated by p42 MAPK in vitro, but is not required for the activation of MAPKAPK-2. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
MAPKAPK-2 (Phospho-T334) polyclonal antibody detects endogenous levels of MAPKAPK-2 protein only when phosphorylated at Thr334. Immunogen :
Synthetic phosphopeptide derived from human MAPKAPK-2 around the phosphorylation site of Threonine 334. Conjugate :
Unconjugated Modification :
Phosphorylation

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  Western blot (WB) analysis of MAPKAPK-2 (Phospho-T334) polyclonal antibody at 1:2000 dilution Lane1:HeLa whole cell lysate(40ug) Lane2:Hela treated with UV 5 min and PMA (200nM,10 min) whole cell lysate(40ug)
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  Immunofluorescence analysis of Hela cells using MAPKAPK-2 (Phospho-T334) antibody at dilution of 1:50.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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