Product Name :
SF1 polyclonal antibody Background :
Mammalian splicing factor 1 (designated SF1, zinc finger protein 162, ZFM1,CW17R and mammalian branch point binding protein [mBBP]) specificallyrecognizes the seven-nucleotide branch point sequence located at 3' splicesites and participates in the assembly of early spliceosomal complexes.Splicing factor 1 functions as a transcriptional repressor and may control bothproliferation and expression of pro-inflammatory gene products in smoothmuscle cells. In addition, cytokine-induced downregulation of splicing factor 1 expression may contribute to the pathogenesis of hyperproliferative inflammatory diseases. The structure of splicing factor 1 contains a nuclear transport domain, a metal binding motif, and glutamine- and proline-rich regions.Human splicing factor 1 also exists as several different isoforms, H1-isoformand Bo-isoform, produced by alternative splicing events. The human splicingfactor 1 gene is located on chromosome 11 close to the gene encoding Menin,the gene responsible for multiple endocrine neoplasia-type 1 (MEN1). Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
SF1 polyclonal antibody detects endogenous levels of SF1 protein. Immunogen :
Synthetic peptide, corresponding to Human SF1. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of SF1 polyclonal antibody at 1:1000 dilution Lane1:A549 whole cell lysate(40ug) Lane2:A2780 whole cell lysate(40ug) Lane3:HEK293T whole cell lysate(40ug)
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  Immunohistochemistry of paraffin-embedded Rat Brain using SF1 antibody at dilution of 1:50.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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