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JNK1/2/3 (phospho-T183/Y185) polyclonal antibody BS4322
  • Western blot (WB) analysis of p-JNK1/2/3 (T183/Y185) polyclonal antibody at 1:500 dilution Lane1:MCF-7 cell lysate treated with UV Lane2:sp2/0 cell lysate treated with UV Lane3:PC12 cell lysate treated with UV
  • Western blot (WB) analysis of JNK1/2/3 (phospho-T183/Y185) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:HEK293T treated with UV (15min) whole cell lysate(40ug) Lane3:NIH-3T3 whole cell lysate(40ug) Lane4:NIH-3T3 treated with UV (15min) whole cell lysate(40ug) Lane5:H9C2 whole cell lysate(40ug) Lane6:H9C2 treated with UV (4h) whole cell lysate(40ug)
  • Western blot (WB) analysis of JNK1/2/3 (phospho-T183/Y185) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:HEK293T treated with UV (15min) whole cell lysate(40ug) Lane3:NIH-3T3 whole cell lysate(40ug) Lane4:NIH-3T3 treated with UV (15min) whole cell lysate(40ug) Lane5:H9C2 whole cell lysate(40ug) Lane6:H9C2 treated with UV (4h) whole cell lysate(40ug)
  • Western blot (WB) analysis of JNK1/2/3 (phospho-T183/Y185) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:HEK293T treated with UV (15min) whole cell lysate(40ug) Lane3:NIH-3T3 whole cell lysate(40ug) Lane4:NIH-3T3 treated with UV (15min) whole cell lysate(40ug) Lane5:H9C2 whole cell lysate(40ug) Lane6:H9C2 treated with UV (4h) whole cell lysate(40ug)
Product NameJNK1/2/3 (phospho-T183/Y185) polyclonal antibody
Catalog No.BS4322
Swiss-ProtP45983/P45984/P53779
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB IF IHC
Application_allWB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
BiowMW~ 46,54 kDa
Alternative NameJNK 46; JNK 55; JNK; JNK1; JNK1A2; JNK2; JNK21B1/2; JNK2ALPHA; JNK2B; JNK2BETA; JNK3; JNK3A; MAP kinase 8; MAP kinase 9; MAP kinase p49 3F12; MAPK 8; MAPK 9; MAPK10; MAPK8; MAPK9; MGC50974; Mitogen activated protein kinase 10; Mitogen activated protein kinase 8; Mitogen activated protein kinase 9
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationPhosphorylation
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100ul $298
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Product Name :
JNK1/2/3 (phospho-T183/Y185) polyclonal antibody
Background :
The human JNK1 (PRKM8, SAPK1, MAPK8) gene maps to chromosome 10q11.22 and shares 83% amino acid identity with JNK2. JNK1 is necessary for normal activation and differentiation of CD4 helper T (TH) cells into TH1 and TH2 effector cells. Capsaicin activates JNK1 and p38 in Ras-transformed human breast epithelial cells. Nitrogen oxides (NOx) upregulate JNK1 in addition to c-Fos, c-Jun and other signaling kinases, including MEKK1 and p38. JNK3 (MK10, MAPK10, PRKM10) is activated by pro-inflammatory cytokines and environmental stresss by phosphorylating transcription factors such as c-Jun and ATF2. This is important for AP-1 transcriptional activity regulation. JNK3 is crucial for neuronal apoptosis (stress-induced).
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
p-JNK1/2/3 (T183/Y185) polyclonal antibody detects endogenous levels of JNK1/2/3 protein only when phosphorylated at Thr183 and Tyr185.
Immunogen :
Synthetic phosphopeptide derived from human JNK1 around the phosphorylation site of Threonine 183 and Tyrosine 185.
Conjugate :
Unconjugated
Modification :
Phosphorylation
  • Western blot (WB) analysis of p-JNK1/2/3 (T183/Y185) polyclonal antibody at 1:500 dilution Lane1:MCF-7 cell lysate treated with UV Lane2:sp2/0 cell lysate treated with UV Lane3:PC12 cell lysate treated with UV
  • Western blot (WB) analysis of JNK1/2/3 (phospho-T183/Y185) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:HEK293T treated with UV (15min) whole cell lysate(40ug) Lane3:NIH-3T3 whole cell lysate(40ug) Lane4:NIH-3T3 treated with UV (15min) whole cell lysate(40ug) Lane5:H9C2 whole cell lysate(40ug) Lane6:H9C2 treated with UV (4h) whole cell lysate(40ug)
  • Western blot (WB) analysis of JNK1/2/3 (phospho-T183/Y185) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:HEK293T treated with UV (15min) whole cell lysate(40ug) Lane3:NIH-3T3 whole cell lysate(40ug) Lane4:NIH-3T3 treated with UV (15min) whole cell lysate(40ug) Lane5:H9C2 whole cell lysate(40ug) Lane6:H9C2 treated with UV (4h) whole cell lysate(40ug)
  • Western blot (WB) analysis of JNK1/2/3 (phospho-T183/Y185) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:HEK293T treated with UV (15min) whole cell lysate(40ug) Lane3:NIH-3T3 whole cell lysate(40ug) Lane4:NIH-3T3 treated with UV (15min) whole cell lysate(40ug) Lane5:H9C2 whole cell lysate(40ug) Lane6:H9C2 treated with UV (4h) whole cell lysate(40ug)
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PMCID:    Pubmed No.:23801677 Protective effect of flavonoid-rich extract from Rosa laevigata Michx on cerebral ischemia–reperfusion injury through suppression of apoptosis and inflammation PMCID:    Pubmed No.:24012531 NF-κB, ERK, p38 MAPK and JNK contribute to the initiation and/or maintenance of mechanical allodynia induced by tumor necrosis factor-alpha in the red nucleus PMCID:    Pubmed No.:24161765 An Apple Oligogalactan Potentiates the Growth Inhibitory Effect of Celecoxib on Colorectal Cancer PMCID:    Pubmed No.:24274457 Proteomic analysis of SP600125-controlled TrkA-dependent targets in SK-N-MC neuroblastoma cells: Inhibition of TrkA activity by SP600125 PMCID:    Pubmed No.:24375967 Rho kinase inhibition by fasudil suppresses lipopolysaccharide-induced apoptosis of rat pulmonary microvascular endothelial cells via JNK and p38 MAPK pathway PMCID:    Pubmed No.:24406296 Protective effects of the total saponins from Dioscorea nipponica Makino against carbon tetrachloride-induced liver injury in mice through suppression of apoptosis and inflammation PMCID:    Pubmed No.:24491258 Dentin matrix proteins (DMPs) enhance differentiation of BMMSCs via ERK and P38 MAPK pathways PMCID:    Pubmed No.:24562313 ZnO nanoparticle-induced oxidative stress triggers apoptosis by activating JNK signaling pathway in cultured primary astrocytes PMCID:    Pubmed No.:24624962 Mineral trioxide aggregate upregulates odonto/osteogenic capacity of bone marrow stromal cells from craniofacial bones via JNK and ERK MAPK signalling pathways PMCID:    Pubmed No.:24635197 Long-term blue light exposure induces RGC-5 cell death in vitro: involvement of mitochondria-dependent apoptosis, oxidative stress, and MAPK signaling pathways PMCID:    Pubmed No.:24682673 Role of cathepsin B in regulating migration and invasion of fibroblast‐like synoviocytes into inflamed tissue from patients with rheumatoid arthritis PMCID:    Pubmed No.:24749816 Effects of sodium fluoride on MAPKs signaling pathway in the gills of a freshwater teleost,< i> Cyprinus carpio PMCID:    Pubmed No.:24780358 Effects of the Total Saponins from Rosa laevigata Michx Fruit against Acetaminophen-Induced Liver Damage in Mice via Induction of Autophagy and Suppression of Inflammation and Apoptosis. 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PMCID:    Pubmed No.:25576701 NF-κB, ERK, p38 MAPK and JNK contribute to the initiation and/or maintenance of mechanical allodynia induced by tumor necrosis factor-alpha in the red nucleus PMCID:    Pubmed No.:24161765 An Apple Oligogalactan Potentiates the Growth Inhibitory Effect of Celecoxib on Colorectal Cancer PMCID:    Pubmed No.:24274457 DGAEE, a newly synthesized derivative of glycyrrhetinic acid, potently attenuates mouse septic shock via its main metabolite DGA in an IL-10-dependent manner PMCID:    Pubmed No.:26456500 Dioscin suppresses human laryngeal cancer cells growth via induction of cell-cycle arrest and MAPK-mediated mitochondrial-derived apoptosis and inhibition of tumor invasion. PMCID:    Pubmed No.:26849940 Role of cathepsin B in regulating migration and invasion of fibroblast‐like synoviocytes into inflamed tissue from patients with rheumatoid arthritis PMCID:    Pubmed No.:24749816 Effects of sodium fluoride on MAPKs signaling pathway in the gills of a freshwater teleost,< i> Cyprinus carpio PMCID:    Pubmed No.:24780358 Proteomic analysis of SP600125-controlled TrkA-dependent targets in SK-N-MC neuroblastoma cells: Inhibition of TrkA activity by SP600125 PMCID:    Pubmed No.:24375967 Rho kinase inhibition by fasudil suppresses lipopolysaccharide-induced apoptosis of rat pulmonary microvascular endothelial cells via JNK and p38 MAPK pathway PMCID:    Pubmed No.:24406296 Protective effects of the total saponins from Dioscorea nipponica Makino against carbon tetrachloride-induced liver injury in mice through suppression of apoptosis and inflammation PMCID:    Pubmed No.:24491258 Dentin matrix proteins (DMPs) enhance differentiation of BMMSCs via ERK and P38 MAPK pathways PMCID:    Pubmed No.:24562313 ZnO nanoparticle-induced oxidative stress triggers apoptosis by activating JNK signaling pathway in cultured primary astrocytes PMCID:    Pubmed No.:24624962 Mineral trioxide aggregate upregulates odonto/osteogenic capacity of bone marrow stromal cells from craniofacial bones via JNK and ERK MAPK signalling pathways PMCID:    Pubmed No.:24635197 Long-term blue light exposure induces RGC-5 cell death in vitro: involvement of mitochondria-dependent apoptosis, oxidative stress, and MAPK signaling pathways PMCID:    Pubmed No.:24682673 Reg3α Overexpression Protects Pancreatic β Cells from Cytokine-Induced Damage and Improves Islet Transplant Outcome PMCID:    Pubmed No.:25826674 The mAb against adipocyte fatty acid-binding protein 2E4 attenuates the inflammation in the mouse model of high-fat diet-induced obesity via toll-like receptor 4 pathway PMCID:    Pubmed No.:25596549 Inhibition of thioredoxin-1 with siRNA exacerbates apoptosis by activating the ASK1-JNK/p38 pathway in brain of a stroke model rats PMCID:    Pubmed No.:25541364 The C-Type Lectin OCILRP2 Costimulates EL4 T Cell Activation via the DAP12-Raf-MAP Kinase Pathway PMCID:    Pubmed No.:25411776 Pretreatment with low dose etomidate prevents etomidate-induced rat adrenal insufficiency by regulating oxidative stress-related MAPKs and apoptosis PMCID:    Pubmed No.:25989534 Downregulation of Foxc2 enhances apoptosis induced by 5-fluorouracil through activation of MAPK and AKT pathways in colorectal cancer PMCID:    Pubmed No.:26893778 Protective effects of Huanglian Wendan Decoction aganist cognitive deficits and neuronal damages in rats with diabetic encephalopathy by inhibiting the release of inflammatory cytokines and repairing insulin signaling pathway in hippocampus PMCID:    Pubmed No.:27914525 Sitagliptin Reduces Endothelial Dysfunction and Apoptosis Induced by High-Fat Diet and Palmitate in Thoracic Aortas and Endothelial Cells via ROS-ER Stress-CHOP Pathway PMCID:    Pubmed No.:34531738 A phycocyanin derived eicosapeptide attenuates lung fibrosis development PMCID:    Pubmed No.:34280398 Downregulation of Siah1 promotes colorectal cancer cell proliferation and migration by regulating AKT and YAP ubiquitylation and proteasome degradation PMCID:    Pubmed No.:32082080 Protective effects of Danzhi jiangtang capsule on vascular endothelial damages induced by high-fat diet and palmitic acid PMCID:    Pubmed No.:30257381 Curcumin restrains hepatic glucose production by blocking cAMP/PKA signaling and reducing acetyl CoA accumulation in high-fat diet (HFD)-fed mice PMCID:    Pubmed No.:29499209 Fuzhuan Brick Tea Boosts Melanogenesis and Prevents Hair Graying through Reduction of Oxidative Stress via NRF2-HO-1 Signaling PMCID:    Pubmed No.:35326249 Inhibition of the antigen-presenting ability of dendritic cells by non-structural protein 2 of influenza A virus PMCID:    Pubmed No.:35259598 Dehydroepiandrosterone protects against oleic acid-triggered mitochondrial dysfunction to relieve oxidative stress and inflammation via activation of the AMPK-Nrf2 axis by targeting GPR30 in hepatocytes PMCID:    Pubmed No.:36773597
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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