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p38 (V318) polyclonal antibody AP0424
  • Western blot (WB) analysis of p38 (V318) polyclonal antibody at 1:1000 dillution Lane1:HEK293T whole cell lysate(36μg) Lane2:NIH-3T3 whole cell lysate(38.8μg) Lane3:HepG2 whole cell lysate(36.8μg)
  • Immunoprecipitation of U-87MG cell lysates using p38 (V318) pAb (Sepharose Bead Conjugate)#BD0048 (lane 3 and lane 4) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 1 and lane 2) .Lane 5 is 30% input. The western blot was probed using p38 (V318) pAb #AP0424.
  • Immunoprecipitation of U-87MG cell lysates using p38 (V318) pAb (Sepharose Bead Conjugate)#BD0048 (lane 3 and lane 4) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 1 and lane 2) .Lane 5 is 30% input. The western blot was probed using p38 (V318) pAb #AP0424.
  • Immunoprecipitation of U-87MG cell lysates using p38 (V318) pAb (Sepharose Bead Conjugate)#BD0048 (lane 3 and lane 4) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 1 and lane 2) .Lane 5 is 30% input. The western blot was probed using p38 (V318) pAb #AP0424.
Product Namep38 (V318) polyclonal antibody
Catalog No.AP0424
Swiss-ProtQ16539
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB IP IF IHC
Application_allWB: 1:1000~1:2000 IHC: 1:50~1:200 IF: 1:50~1:200 IP: 1:50~1:200
BiowMW~ 42 kDa
Alternative NameMitogen-activated protein kinase 14; MAP kinase 14; MAPK 14; Cytokine suppressive anti-inflammatory drug-binding protein; CSAID-binding protein; CSBP; MAP kinase MXI2; MAX-interacting protein 2; Mitogen-activated protein kinase p38 alpha; MAP kinase p38 alpha; Stress-activated protein kinase 2a; SAPK2a; MAPK14; CSBP; CSBP1; CSBP2; CSPB1; MXI2; SAPK2A
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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50ul $158
100ul $275
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Product Name :
p38 (V318) polyclonal antibody
Background :
MAP (mitogen-activated protein) kinases play a significant role in many biological processes, including cell adhesion and spreading, cell differentiation and apoptosis. p38α, p38β and p38γ, also known as MAPK14, MAPK11 and MAPK12, respectively, each contain one protein kinase domain and belong to the MAP kinase family. Expressed in different areas throughout the body with common expression patterns in heart, p38 proteins use magnesium as a cofactor to catalyze the ATP-dependent phosphorylation of target proteins. Via their catalytic activity, p38α, p38β and p38γ are involved in a variety of events throughout the cell, including signal transduction pathways, cytokine production and cell proliferation and differentiation.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
p38 (V318) polyclonal antibody detects endogenous levels of p38 protein.
Immunogen :
Synthetic peptide, corresponding to Human p38.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot (WB) analysis of p38 (V318) polyclonal antibody at 1:1000 dillution Lane1:HEK293T whole cell lysate(36μg) Lane2:NIH-3T3 whole cell lysate(38.8μg) Lane3:HepG2 whole cell lysate(36.8μg)
  • Immunoprecipitation of U-87MG cell lysates using p38 (V318) pAb (Sepharose Bead Conjugate)#BD0048 (lane 3 and lane 4) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 1 and lane 2) .Lane 5 is 30% input. The western blot was probed using p38 (V318) pAb #AP0424.
  • Immunoprecipitation of U-87MG cell lysates using p38 (V318) pAb (Sepharose Bead Conjugate)#BD0048 (lane 3 and lane 4) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 1 and lane 2) .Lane 5 is 30% input. The western blot was probed using p38 (V318) pAb #AP0424.
  • Immunoprecipitation of U-87MG cell lysates using p38 (V318) pAb (Sepharose Bead Conjugate)#BD0048 (lane 3 and lane 4) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 1 and lane 2) .Lane 5 is 30% input. The western blot was probed using p38 (V318) pAb #AP0424.
Antidiabetic Effect of Oleanolic Acid: A Promising Use of a Traditional Pharmacological Agent PMCID:    Pubmed No.:21254272 Double antioxidant activities of rosiglitazone against high glucose-induced oxidative stress in hepatocyte PMCID:    Pubmed No.:21333731 ROS-activated p38 MAPK/ERK-Akt cascade plays a central role in palmitic acid-stimulated hepatocyte proliferation. PMCID:    Pubmed No.:21620957 Methyl-1-hydroxy-2-naphthoate, a novel naphthol derivative, inhibits lipopolysaccharide-induced inflammatory response in macrophages via suppression of NF-jB, JNK and p38 MAPK pathways PMCID:    Pubmed No.:21667204 XJP-1, a novel ACEI, with anti-inflammatory properties in HUVECs PMCID:    Pubmed No.:21803355 Insulin-like growth factor 1 enhances the proliferation and osteogenic differentiation of human periodontal ligament stem cells via ERK and JNK MAPK pathways PMCID:    Pubmed No.:22227802 Madecassoside suppresses migration of fibroblasts from keloids: involvement of p38 kinase and PI3K signaling pathways PMCID:    Pubmed No.:22360962 Disruption of mindin exacerbates cardiac hypertrophy and fibrosis PMCID:    Pubmed No.:22367478 Suppression of endothelial cell adhesion by XJP-1, a new phenolic compound derived from banana peel PMCID:    Pubmed No.:22609942 Diosgenin down-regulates NF-κB p65/p50 and p38MAPK pathways and attenuates acute lung injury induced by lipopolysaccharide in mice PMCID:    Pubmed No.:23246979 Dietary flavonoid genistein induces Nrf2 and phase II detoxification gene expression via ERKs and PKC pathways and protects against oxidative stress in Caco-2 cells PMCID:    Pubmed No.:23255485 5,7-Dihydroxyflavone Enhances the Apoptosis-Inducing Potential of TRAIL in Human Tumor Cells via Regulation of Apoptosis-Related Proteins PMCID:    Pubmed No.:23533482 An apple oligogalactan suppresses endotoxin-induced cyclooxygenase-2 expression by inhibition of LPS pathways PMCID:    Pubmed No.:23827762 Induction of apoptosis and inhibition of invasion in choriocarcinoma JEG-3 cells by α-calendic acid and β-calendic acid PMCID:    Pubmed No.:24035100 Musashi2 modulates K562 leukemic cell proliferation and apoptosis involving the MAPK pathway PMCID:    Pubmed No.:24076374 Protective effects of dioscin against alcohol-induced liver injury PMCID:    Pubmed No.:24146112 An Apple Oligogalactan Potentiates the Growth Inhibitory Effect of Celecoxib on Colorectal Cancer PMCID:    Pubmed No.:24274457 Role of p38, ERK1/2, focal adhesion kinase, RhoA/ROCK and cytoskeleton in the adipogenesis of human mesenchymal stem cells. PMCID:    Pubmed No.:2433197 Rho kinase inhibition by fasudil suppresses lipopolysaccharide-induced apoptosis of rat pulmonary microvascular endothelial cells via JNK and p38 MAPK pathway PMCID:    Pubmed No.:24406296 LW-214, a newly synthesized flavonoid, induces intrinsic apoptosis pathway by down-regulating Trx-1 in MCF-7 human breast cells PMCID:    Pubmed No.:24374359 Protective effects of the total saponins from Dioscorea nipponica Makino against carbon tetrachloride-induced liver injury in mice through suppression of apoptosis and inflammation PMCID:    Pubmed No.:24491258 The DPP-4 inhibitor sitagliptin attenuates the progress of atherosclerosis in apolipoprotein-E-knockout mice via AMPK-and MAPK-dependent mechanisms PMCID:    Pubmed No.:24490809 Dentin matrix proteins (DMPs) enhance differentiation of BMMSCs via ERK and P38 MAPK pathways PMCID:    Pubmed No.:24562313 ZnO nanoparticle-induced oxidative stress triggers apoptosis by activating JNK signaling pathway in cultured primary astrocytes PMCID:    Pubmed No.:24624962 Role of cathepsin B in regulating migration and invasion of fibroblast‐like synoviocytes into inflamed tissue from patients with rheumatoid arthritis PMCID:    Pubmed No.:24749816 Effects of the Total Saponins from Rosa laevigata Michx Fruit against Acetaminophen-Induced Liver Damage in Mice via Induction of Autophagy and Suppression of Inflammation and Apoptosis. PMCID:    Pubmed No.:24886943 Diosgenin inhibits superoxide generation in FMLP-activated mouse neutrophils via multiple pathways PMCID:    Pubmed No.:25246240 IL‐17 regulates the expressions of RANKL and OPG in human periodontal ligament cells via TRAF6/TBK1‐JNK/NF‐κB pathways PMCID:    Pubmed No.:25263088 Arctigenin but not arctiin acts as the major effective constituent of Arctium lappa L. fruit for attenuating colonic inflammatory response induced by dextran sulfate sodium in mice. (Application: Mouse WB) PMCID:    Pubmed No.:25284342 Potent Effects of the Total Saponins from Dioscorea nipponica Makino Against Streptozotocin-Induced Type 2 Diabetes Mellitus in Rats. PMCID:    Pubmed No.:25322985 The protective effects of urocortin1 against intracerebral hemorrhage by activating JNK1/2 and p38 phosphorylation and further increasing VEGF via corticotropin-releasing factor receptor 2. PMCID:    Pubmed No.:25576701 Role of the MAPK pathway in the observed bystander effect in lymphocytes co-cultured with macrophages irradiated with γ-rays or carbon ions PMCID:    Pubmed No.:25748424 Protective effects of protocatechuic acid on acute lung injury induced by lipopolysaccharide in mice via p38MAPK and NF-κB signal pathways PMCID:    Pubmed No.:25841318 Epigallocatechin-3-gallate attenuates lipopolysaccharide-induced mastitis in rats via suppressing MAPK mediated inflammatory responses and oxidative stress PMCID:    Pubmed No.:25840281 PM2. 5‐induced oxidative stress increases adhesion molecules expression in human endothelial cells through the ERK/AKT/NF‐κB‐dependent pathway PMCID:    Pubmed No.:25876056 Ruscogenin suppresses mouse neutrophil activation: Involvement of protein kinase A pathway PMCID:    Pubmed No.:26134424 Protective effect of butyrate against ethanol-induced gastric ulcers in mice by promoting the anti-inflammatory, anti-oxidant and mucosal defense mechanisms PMCID:    Pubmed No.:26604089 Phloretin induces apoptosis of non-small cell lung carcinoma A549 cells via JNK1/2 and p38 MAPK pathways PMCID:    Pubmed No.:26503828 Norisoboldine ameliorates DSS-induced ulcerative colitis in mice through induction of regulatory T cells in colons. PMCID:    Pubmed No.:26363976 DGAEE, a newly synthesized derivative of glycyrrhetinic acid, potently attenuates mouse septic shock via its main metabolite DGA in an IL-10-dependent manner PMCID:    Pubmed No.:26456500 Dioscin suppresses human laryngeal cancer cells growth via induction of cell-cycle arrest and MAPK-mediated mitochondrial-derived apoptosis and inhibition of tumor invasion. PMCID:    Pubmed No.:26849940 Diosgenin inhibits superoxide generation in FMLP-activated mouse neutrophils via multiple pathways PMCID:    Pubmed No.:25246240 IL‐17 regulates the expressions of RANKL and OPG in human periodontal ligament cells via TRAF6/TBK1‐JNK/NF‐κB pathways PMCID:    Pubmed No.:25263088 Arctigenin but not arctiin acts as the major effective constituent of Arctium lappa L. fruit for attenuating colonic inflammatory response induced by dextran sulfate sodium in mice. (Application: Mouse WB) PMCID:    Pubmed No.:25284342 Potent Effects of the Total Saponins from Dioscorea nipponica Makino Against Streptozotocin-Induced Type 2 Diabetes Mellitus in Rats. PMCID:    Pubmed No.:25322985 The protective effects of urocortin1 against intracerebral hemorrhage by activating JNK1/2 and p38 phosphorylation and further increasing VEGF via corticotropin-releasing factor receptor 2. PMCID:    Pubmed No.:25576701 Musashi2 modulates K562 leukemic cell proliferation and apoptosis involving the MAPK pathway PMCID:    Pubmed No.:24076374 Protective effects of dioscin against alcohol-induced liver injury PMCID:    Pubmed No.:24146112 An Apple Oligogalactan Potentiates the Growth Inhibitory Effect of Celecoxib on Colorectal Cancer PMCID:    Pubmed No.:24274457 Protective effect of butyrate against ethanol-induced gastric ulcers in mice by promoting the anti-inflammatory, anti-oxidant and mucosal defense mechanisms PMCID:    Pubmed No.:26604089 Phloretin induces apoptosis of non-small cell lung carcinoma A549 cells via JNK1/2 and p38 MAPK pathways PMCID:    Pubmed No.:26503828 Norisoboldine ameliorates DSS-induced ulcerative colitis in mice through induction of regulatory T cells in colons. PMCID:    Pubmed No.:26363976 DGAEE, a newly synthesized derivative of glycyrrhetinic acid, potently attenuates mouse septic shock via its main metabolite DGA in an IL-10-dependent manner PMCID:    Pubmed No.:26456500 Dioscin suppresses human laryngeal cancer cells growth via induction of cell-cycle arrest and MAPK-mediated mitochondrial-derived apoptosis and inhibition of tumor invasion. PMCID:    Pubmed No.:26849940 Role of cathepsin B in regulating migration and invasion of fibroblast‐like synoviocytes into inflamed tissue from patients with rheumatoid arthritis PMCID:    Pubmed No.:24749816 Role of p38, ERK1/2, focal adhesion kinase, RhoA/ROCK and cytoskeleton in the adipogenesis of human mesenchymal stem cells. PMCID:    Pubmed No.:2433197 Rho kinase inhibition by fasudil suppresses lipopolysaccharide-induced apoptosis of rat pulmonary microvascular endothelial cells via JNK and p38 MAPK pathway PMCID:    Pubmed No.:24406296 LW-214, a newly synthesized flavonoid, induces intrinsic apoptosis pathway by down-regulating Trx-1 in MCF-7 human breast cells PMCID:    Pubmed No.:24374359 Protective effects of the total saponins from Dioscorea nipponica Makino against carbon tetrachloride-induced liver injury in mice through suppression of apoptosis and inflammation PMCID:    Pubmed No.:24491258 The DPP-4 inhibitor sitagliptin attenuates the progress of atherosclerosis in apolipoprotein-E-knockout mice via AMPK-and MAPK-dependent mechanisms PMCID:    Pubmed No.:24490809 Dentin matrix proteins (DMPs) enhance differentiation of BMMSCs via ERK and P38 MAPK pathways PMCID:    Pubmed No.:24562313 ZnO nanoparticle-induced oxidative stress triggers apoptosis by activating JNK signaling pathway in cultured primary astrocytes PMCID:    Pubmed No.:24624962 Tris (2-chloroethyl) phosphate induces senescence-like phenotype of hepatocytes via the p21Waf1/Cip1-Rb pathway in a p53-independent manner PMCID:    Pubmed No.:28886428 Inhibition of thioredoxin-1 with siRNA exacerbates apoptosis by activating the ASK1-JNK/p38 pathway in brain of a stroke model rats PMCID:    Pubmed No.:25541364 A phycocyanin derived eicosapeptide attenuates lung fibrosis development PMCID:    Pubmed No.:34280398 Estrogen promotes the metastasis of non‑small cell lung cancer via estrogen receptor β by upregulation of Toll‑like receptor 4 and activation of the myd88/NF‑κB/MMP2 pathway PMCID:    Pubmed No.:32364243
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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