Product Name :
DDDDK-tag polyclonal antibody Background :
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
DDDDK-tag polyclonal antibody detects over-expressed or recombinant proteins containing the DDDDK epitope tag. Immunogen :
Synthetic peptide DDDDK-tag Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of DDDDK-tag pAb at 1:5000 dillution Lane1:HEK293T whole cell lysate Lane2:HEK293T whole cell lysate,transfected with pcDNA3.1-DDDDK-p62-HA.
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  IF image of AP0007 stained HEK293T cells, transfected with pcDNA3.1-HA-DDDDK-p62 #PPL00549-2b. The cells were 4% paraformaldehyde fixed (20 min) and then incubated in 10% normal goat serum for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody DDDDK-tag pAb #AP0007(1:200) and the antibody HA-tag (4G3) mAb #AP0005M(1:200) at 5µg/ml overnight at +4°C. The secondary antibody (Green) wasGoat anti-Rabbit IgG (H+L) -FITC#BS10950 and the secondary antibody (Red) was Goat anti-Mouse IgG (H+L)-TRITC#BS11502. Hoechst33342 #BD5011 was used to stain the cell nuclei (blue).
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  IF image of AP0007 stained HEK293T cells, transfected with pcDNA3.1-HA-DDDDK-p62 #PPL00549-2b. The cells were 4% paraformaldehyde fixed (20 min) and then incubated in 10% normal goat serum for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody DDDDK-tag pAb #AP0007(1:200) and the antibody HA-tag (4G3) mAb #AP0005M(1:200) at 5µg/ml overnight at +4°C. The secondary antibody (Green) wasGoat anti-Rabbit IgG (H+L) -FITC#BS10950 and the secondary antibody (Red) was Goat anti-Mouse IgG (H+L)-TRITC#BS11502. Hoechst33342 #BD5011 was used to stain the cell nuclei (blue).

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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