Product Name :
Claudin 18 monoclonal antibody Background :
The Claudin superfamily consists of many structurally related proteins in humans . These proteins are important structural and functional components of tight junctions in paracellular transport . Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues . Three classes of proteins are known to localize to tight junctions, including the Claudins, Occludin and Junction adhesion molecule . Claudins, which consist of four transmembrane domains and two extracellular loops make up tight junction strands . Emerging evidence suggests that the Claudin family of proteins regulates transport through tight junctions via differential discrimination for solute size and charge .Claudin expression is often highly restricted to specfic regions of different tissues and may have an important role in transcellular transport through tight junctions . Product :
Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Recognizes endogenous levels of Claudin 18 protein. Immunogen :
KLH-conjugated synthetic peptide encompassing a sequence within human Claudin 18. The exact sequence is proprietary. Conjugate :
Unconjugated Modification :
Unmodification

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  Immunohistochemical analysis of Claudin 18 staining in human stomach formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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